EXPRESSION OF P2X5 RECEPTORS IN THE MOUSE CNS
EXPRESSION OF P2X5 RECEPTORS IN THE MOUSE CNS
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DOI：
10.1016/j.neuroscience.2008.07.062
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发表时间：
2008-10-15
期刊：
影响因子：
3.3
通讯作者：
Xiang, Z.
中科院分区：
文献类型：
--
作者：
Guo, W.;Xu, X.;Xiang, Z.
P2X受体是由七个已知亚基(P2X(1-7))组成的ATP门控阳离子通道，它们在神经组织中参与不同的功能。本研究用免疫组织化学和原位杂交组织化学方法研究了小鼠中枢神经系统中P2X(5)受体的表达模式。免疫染色的特异性已通过预吸收、Western印迹和原位杂交方法得到验证。在嗅球、大脑皮质、苍白球、杏仁前皮质、端脑杏仁海马区；丘脑前核、前腹核、腹外侧核；视上核、腹内侧核、弓状核；中脑黑质；桥核、中脑三叉神经核、三叉神经运动核、疑核、下橄榄核、舌下核、迷走神经背侧核、后脑后区、小脑浦肯野细胞、脊髓等部位有较强的P2X(5)受体表达。小鼠中枢神经系统内广泛的P2X(5)受体免疫反应性和mRNA分布与细胞外ATP作为快速神经递质的作用是一致的。(C)2008年IBRO。爱思唯尔有限公司出版。保留所有权利。
P2X receptors are ATP-gated cationic channels composed of seven known subunits (P2X(1-7)) which are involved in different functions in neural tissue. The present study investigates the P2X(5) receptor expression pattern in the mouse CNS using immunohistochemistry and in situ hybridization histochemistry. The specificity of the immunostaining has been verified by pre-absorption, Western blot and in situ hybridization methods. Heavy P2X(5) receptor immunostaining was observed in the mitral cells of the olfactory bulb; cerebral cortex; globus pallidum, anterior cortical amygdaloid nucleus, amygdalohippocampal area of subcortical telencephalon; anterior nuclei, anteroventral nucleus, ventrolateral nucleus of thalamus; supraoptic nucleus, ventromedial nucleus, arcuate nucleus of hypothalamus; substantia nigra of midbrain; pontine nuclei, mesencephalic trigeminal nucleus, motor trigeminal nucleus, ambiguous nucleus, inferior olive, hypoglossal nucleus, dorsal motor vagus nucleus, area postrema of hindbrain; Purkinje cells of cerebellum; and spinal cord. The identification of extensive P2X(5) receptor immunoreactivity and mRNA distribution within the CNS of the mouse demonstrated here is consistent with a role for extracellular ATP acting as a fast neurotransmitter. (C) 2008 IBRO. Published by Elsevier Ltd. All rights reserved.