In planta secretion of a calreticulin by migratory and sedentary stages of root-knot nematode
In planta secretion of a calreticulin by migratory and sedentary stages of root-knot nematode
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DOI：
10.1094/mpmi-18-1277
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发表时间：
2005-12-01
影响因子：
3.5
通讯作者：
Rosso, MN
中科院分区：
文献类型：
--
作者：
Jaubert, S;Milac, AL;Rosso, MN
内寄生定居线虫的食道分泌物被认为在整个植物寄生过程中起着关键作用，特别是在根组织的入侵和线虫发育所必需的线虫取食位点（NITS）的启动和维持期间。在植物中分泌的食管细胞壁降解酶的迁移青少年已被证明，这表明这些酶在入侵阶段的作用。然而，食管腺蛋白分泌到NFS的线虫定居阶段从未被证明。钙网蛋白Mi-CRT是在南方根结线虫的食道腺中合成的蛋白质。在对MiCRT蛋白进行三维建模后，选择表面肽来产生特异性抗体。在植物免疫定位表明，Mi-CRT分泌的迁移和定居阶段的线虫，表明Mi-CRT在整个寄生的作用。在NITS的维持过程中，分泌的Mi-CRT定位于线虫的外部，位于口针的尖端。此外，观察到Mi-CRT沿着构成摄食部位的巨细胞的细胞壁沿着蓄积，为线虫食管蛋白分泌到NITS中提供了证据。
Esophageal secretions from endoparasitic sedentary nematodes are thought to play key roles throughout plant parasitism, in particular during the invasion of the root tissue and the initiation and maintenance of the nematode feeding site (NITS) essential for nematode development. The secretion in planta of esophageal cell-wall-degrading enzymes by migratory juveniles has been shown, suggesting a role for these enzymes in the invasion phase. Nevertheless, the secretion of an esophageal gland protein into the NFS by nematode sedentary stages has never been demonstrated. The calreticulin Mi-CRT is a protein synthesized in the esophageal glands of the root-knot nematode Meloidogyne incognita. After three-dimensional modeling of the MiCRT protein, a surface peptide was selected to raise specific antibodies. In planta immunolocalization showed that Mi-CRT is secreted by migratory and sedentary stage nematodes, suggesting a role for Mi-CRT throughout parasitism. During the maintenance of the NITS, the secreted Mi-CRT was localized outside the nematode at the tip of the stylet. In addition, Mi-CRT accumulation was observed along the cell wall of the giant cells that compose the feeding site, providing evidence for a nematode esophageal protein secretion into the NITS.