Mechanistic aspects of CPP-mediated intracellular drug delivery:: Relevance of CPP self-assembly
Mechanistic aspects of CPP-mediated intracellular drug delivery:: Relevance of CPP self-assembly
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DOI：
10.1016/j.bbamem.2006.01.006
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发表时间：
2006-03-01
影响因子：
3.4
通讯作者：
Giralt, Ernest
中科院分区：
文献类型：
--
作者：
Pujals, Silvia;Fernandez-Carneado, Jimena;Giralt, Ernest
近年来，细胞穿透肽已被证明是一种有效的细胞内递送系统。CPP内化的机制首先涉及与细胞外基质的相互作用，在大多数情况下随后是内吞作用，最后，根据内吞作用的类型，达到细胞内命运。连接到CPP的货物的递送需要内体释放，最近已经提出了不同的方法。带正电荷的氨基酸、疏水性和/或两亲性是CPP所共有的。此外，一些CPP可以自组装。本文讨论了自组装在细胞摄取CPP中的作用。甜箭肽（SAP）CPP已通过CD和TEM（冷冻固定/冷冻干燥）显示出聚集体，尽管内化物质尚未被鉴定为单体或聚集体。(c)2006 Elsevier B. V.保留所有权利。
In recent years, cell-penetrating peptides have proven to be an efficient intracellular delivery system. The mechanism for CPP internalisation, which first involves interaction with the extracellular matrix, is followed in most cases by endocytosis and finally, depending on the type of endocytosis, an intracellular fate is reached. Delivery of cargo attached to a CPP requires endosomal release, for which different methods have recently been proposed. Positively charged amino acids, hydrophobicity and/or amphipathicity are common to CPPs. Moreover, some CPPs can self-assemble. Herein is discussed the role of self assembly in the cellular uptake of CPPs. Sweet Arrow Peptide (SAP) CPP has been shown to aggregate by CD and TEM (freeze-fixation/freeze-drying), although the internalised species have yet to be identified as either the monomer or an aggregate. (c) 2006 Elsevier B.V. All rights reserved.