Draft genome of the filarial nematode parasite Brugia malayi
Draft genome of the filarial nematode parasite Brugia malayi
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DOI：
10.1126/science.1145406
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发表时间：
2007-09-21
期刊：
影响因子：
56.9
通讯作者：
Scott, Alan L.
中科院分区：
文献类型：
--
作者：
Ghedin, Elodie;Wang, Shiliang;Scott, Alan L.
引起象皮病和河盲症的寄生线虫威胁着发展中国家数亿人的生命。我们已经测序了人类丝虫寄生虫马来丝虫（Brugia malayi）的90 Mb基因组，并预测在71 Mb的稳健组装序列中有11，500个蛋白质编码基因。与自由生活的模式线虫秀丽隐杆线虫的比较分析表明，尽管这些基因在近似3.5亿年的进化过程中几乎没有保持局部同线性的保守性，但它们在很大程度上仍然保持在染色体单位上的连锁。鉴定了100多个保守的操纵子。对预测蛋白质组的分析为B的适应性提供了证据。马来人在其人类和载体宿主的壁龛和深入了解与其沃尔巴克氏体内共生体的互利关系的分子基础。这些发现为合理的药物设计提供了基础。
Parasitic nematodes that cause elephantiasis and river blindness threaten hundreds of millions of people in the developing world. We have sequenced the similar to 90 megabase (Mb) genome of the human filarial parasite Brugia malayi and predict similar to 11,500 protein coding genes in 71 Mb of robustly assembled sequence. Comparative analysis with the free-living, model nematode Caenorhabditis elegans revealed that, despite these genes having maintained little conservation of local synteny during similar to 350 million years of evolution, they largely remain in linkage on chromosomal units. More than 100 conserved operons were identified. Analysis of the predicted proteome provides evidence for adaptations of B. malayi to niches in its human and vector hosts and insights into the molecular basis of a mutualistic relationship with its Wolbachia endosymbiont. These findings offer a foundation for rational drug design.