Zebrafish (Danio rerio) Is an Economical and Efficient Animal Model for Screening Potential Anti-cataract Compounds.
Zebrafish (Danio rerio) Is an Economical and Efficient Animal Model for Screening Potential Anti-cataract Compounds.
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DOI：
10.1167/tvst.11.8.21
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发表时间：
2022-08-01
影响因子：
3
通讯作者：
Chen, Wei-Yi
中科院分区：
文献类型：
--
作者：
Liu, Chun-Fu;Ou-Yang, Yen;Huang, Ching-Ying;Jao, Shih-Wei;Kuo, Yu-Kai;Chen, Hung-Chi;Cheng, Shu-Chun;Wang, Nan-Kai;Chuang, Lan-Hsin;Chen, Yau-Hung;Chen, Wei-Yi
建立斑马鱼白内障动物模型，用于筛选潜在的抗白内障药物。活体斑马鱼被麻醉，并暴露于紫外线-C（UV-C）照射，剂量为3250 mJ/cm 2/d，直到他们发展成严重的白内障。根据ImageQuant TL 7.0版分析的照片对这些白内障进行分级。患有严重白内障的鱼被用来评估一系列用于白内障治疗的化合物，包括先前证明的热门化合物羊毛甾醇。为了进行初步评估，将鱼分为四组：未处理组、平衡盐溶液组、β-环糊精（β-CD）组和溶解在β-CD中的羊毛固醇组。处理进行10天，并评价镜片的透明度。为了评估处理的持久性，连续7天用β-CD和溶于β-CD的羊毛甾醇处理鱼，然后监测3天不处理。使用目前的UV-C照射方案，斑马鱼患严重白内障的平均时间为7.8天（范围4 - 15天）。两项研究设计都只需要另外10天来确定命中化合物的效果。整个试验周期可在一个月内完成，整个试验经济合理。我们可以用这种新开发的斑马鱼白内障模型在短时间内以合理的成本测定大量的命中化合物。这些测定可以允许开发用于筛选潜在的抗白内障化合物的有效平台。这些结果可能有助于在进一步的实验和研究后开发用于人类的抗白内障药物。
To develop a zebrafish cataract model for screening potential anti-cataract compounds. Living zebrafish were anesthetized and exposed to ultraviolet-C (UV-C) irradiation at a dosage of 3250 mJ/cm2/d until they developed severe cataracts. These cataracts were graded based on photographs analyzed with ImageQuant TL version 7.0. Fish with severe cataracts were used to evaluate a range of compounds for cataract treatment, including the previously demonstrated hit compound lanosterol. For the initial evaluation, fish were divided into four groups: no treatment, balanced salt solution, β-cyclodextrin (β-CD), and lanosterol dissolved in β-CD. The treatments were performed for 10 days, and the clarity of lenses was evaluated. To assess the persistence of treatment, fish were treated with β-CD and lanosterol dissolved in β-CD for seven consecutive days followed by monitoring for three days without treatment. The average time for zebrafish to develop severe cataracts using the present UV-C irradiation protocol was 7.8 days (range 4–15 days). Both study designs required only another 10 days to determine the effect of hit compounds. The total experimental period could be completed within one month, and the entire experiment was economical. We could assay a large number of hit compounds at a reasonable cost and within a short time using this newly developed zebrafish cataract model. These assays may allow development of an efficient platform for screening potential anti-cataract compounds. The results may facilitate the development of ani-cataract medication for humans after further experiments and investigations.
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多个上游模块调节斑马鱼MyF5表达式。
影响因子：
--
作者：
Chen, Yau-Hung;Wang, Yun-Hsin;Chang, Min-Yen;Lin, Cheng-Yung;Weng, Chih-Wei;Westerfield, Monte;Tsai, Huai-Jen
通讯作者：
Tsai, Huai-Jen
影响因子：
13.6
作者：
Moreau KL;King JA
通讯作者：
King JA
DOI：
10.1126/science.aac9145
发表时间：
2015-11-06
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Makley LN;McMenimen KA;DeVree BT;Goldman JW;McGlasson BN;Rajagopal P;Dunyak BM;McQuade TJ;Thompson AD;Sunahara R;Klevit RE;Andley UP;Gestwicki JE
通讯作者：
Gestwicki JE
影响因子：
3.9
作者：
Li, Xiao-Qiao;Cai, Hong-Chen;Ma, Xu
通讯作者：
Ma, Xu
影响因子：
3.9
作者：
Falavarjani, K. Ghasemi;Nguyen, Q. D.
通讯作者：
Nguyen, Q. D.