Pseudomonas sax Genes Overcome Aliphatic Isothiocyanate-Mediated Non-Host Resistance in Arabidopsis
Pseudomonas sax Genes Overcome Aliphatic Isothiocyanate-Mediated Non-Host Resistance in Arabidopsis
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DOI：
10.1126/science.1199707
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发表时间：
2011-03-04
期刊：
影响因子：
56.9
通讯作者：
Lamb, Chris
中科院分区：
文献类型：
--
作者：
Fan, Jun;Crooks, Casey;Lamb, Chris
大多数植物与微生物的相互作用不会导致疾病；天然产物限制非宿主病原体。我们发现萝卜硫素（4-甲基亚磺酰基丁基异硫氰酸酯）是一种源自脂肪族硫代葡萄糖苷的天然产物，可抑制拟南芥植物中非宿主假单胞菌的生长。在对拟南芥有毒力的假单胞菌中鉴定出多个 sax 基因 (saxCAB/F/D/G)。这些 sax 基因需要压倒基于异硫氰酸盐的防御并促进疾病结果，特别是在对植物生存至关重要的幼叶中。将 saxCAB 基因引入非宿主菌株使它们能够克服这些拟南芥防御。我们的研究表明，脂肪族异硫氰酸酯（之前已被证明可以限制食草动物的损害）也是至关重要的、强大的、发育调节的防御，是拟南芥-假单胞菌病理系统中非宿主抗性的基础。
Most plant-microbe interactions do not result in disease; natural products restrict non-host pathogens. We found that sulforaphane (4-methylsulfinylbutyl isothiocyanate), a natural product derived from aliphatic glucosinolates, inhibits growth in Arabidopsis of non-host Pseudomonas bacteria in planta. Multiple sax genes (saxCAB/F/D/G) were identified in Pseudomonas species virulent on Arabidopsis. These sax genes are required to overwhelm isothiocyanate-based defenses and facilitate a disease outcome, especially in the young leaves critical for plant survival. Introduction of saxCAB genes into non-host strains enabled them to overcome these Arabidopsis defenses. Our study shows that aliphatic isothiocyanates, previously shown to limit damage by herbivores, are also crucial, robust, and developmentally regulated defenses that underpin non-host resistance in the Arabidopsis-Pseudomonas pathosystem.