Antimicrobial activity of cuprous oxide-coated and cupric oxide-coated surfaces
Antimicrobial activity of cuprous oxide-coated and cupric oxide-coated surfaces
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DOI：
10.1016/j.jhin.2022.07.022
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发表时间：
2022-10-05
影响因子：
6.9
通讯作者：
Duckera, W. A.
中科院分区：
文献类型：
--
作者：
Behzadinasaba, S.;Hosseinia, M.;Duckera, W. A.
背景：疾病可通过接触受污染的表面传播。例如，变形菌与耐甲氧西林金黄色葡萄球菌(MRSA)和铜绿假单胞菌的传播有关。目的：检测氧化亚铜(Cu2O)和氧化铜(CuO)涂层对包括细菌和真菌在内的12种微生物的抗菌活性。Cu2O涂层的制备采用简单的两步法，即用聚氨酯粘结活性氧化铜颗粒；将Cu2O颗粒在空气中进行热处理，生成氧化铜(CuO)，并进行早期烧结，形成连续涂层。用10毫升微生物悬浮液检测其抗菌活性，然后以细胞为菌落形成单位(CFU)进行计数。结果：该涂层可迅速杀灭9种不同的微生物，包括革兰氏阴性和革兰氏阳性细菌、分枝杆菌和真菌。例如，Cu2O/PU涂层在1小时内对铜绿假单胞菌和金黄色葡萄球菌的杀灭率分别为99.9997%和99.9993%，每周清洗后效果没有下降。经过磨损处理后的Cu2O涂层的抗菌活性没有变化，并且对人体细胞没有毒性。结论：Cu2O涂层的广谱抗菌活性、耐磨性和低毒性的结合表明了其在医疗保健领域的潜在应用。(C)2022医疗感染学会。爱思唯尔有限公司出版。保留所有权利。
Background: Disease can be spread through contact with contaminated surfaces (fomites). For example, fomites have been implicated in the spread of meticillin-resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa. Antimicrobial surface treatments are a potential method of reducing disease transmission from fomites, and broad-spectrum activity is desirable.Aim: To test cuprous oxide (Cu2O) and cupric oxide (CuO) coatings for antimicrobial activity against 12 micro-organisms including bacteria and fungi.Methods: We fabricated two surface coatings. The Cu2O coating was fabricated in a simple two-step process using polyurethane to bind the active copper oxide particles; CuO was prepared by heat treatment of Cu2O particles in air to produce cupric oxide (CuO) and to cause early-stage sintering to form a continuous coating. The antimicrobial activity was examined with 10 mL of microbial suspension droplets followed by counting cells as colony -forming units (cfu).Findings: The coatings rapidly killed nine different micro-organisms, including Gram-negative and Gram-positive bacteria, mycobacteria and fungi. For example, the Cu2O/ PU coating killed 99.9997% of P. aeruginosa and 99.9993% of S. aureus after 1 h. Efficacy was not reduced after weekly cleanings. The antimicrobial activity of the Cu2O coating was unchanged after abrasion treatment, and the coatings were not cytotoxic to human cells.Conclusion: The combination of broad-spectrum antimicrobial activity, abrasion resist-ance, and low toxicity of the Cu2O coating suggests potential use in healthcare settings.(c) 2022 The Healthcare Infection Society. Published by Elsevier Ltd. All rights reserved.