Identification, cloning and characterization of interleukin-17 and its family from zebrafish
Identification, cloning and characterization of interleukin-17 and its family from zebrafish
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DOI：
10.1016/j.fsi.2006.01.004
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发表时间：
2006-10-01
影响因子：
4.7
通讯作者：
Sakai, Masahiro
中科院分区：
文献类型：
--
作者：
Gunimaladevi, I.;Savan, Ram;Sakai, Masahiro
细胞因子是参与免疫的主要信号分子之一。这些细胞因子中的许多已在脊椎动物中分离出来，并发现在宿主防御机制中发挥重要作用。白细胞介素-17（IL-17）家族的基因已知具有促炎作用并且与特定疾病状况相关，这些基因在脊椎动物进化中是保守的。在这项研究中，斑马鱼（Danio rerio）基因组的计算机筛选导致了5个携带IL-17基因的斑马鱼的鉴定。5个IL-17基因的同源性为19.3%~ 61.9%。这些分子显示出半胱氨酸的保守性，这对于IL-17分子的二硫键很重要。除IL-17 D基因只有一个内含子和两个外显子外，其余基因的结构组成均为两个内含子和三个外显子。采用最大简约法进行系统发育分析，结果表明斑马鱼IL-17基因与其他IL-17同源基因聚类性较好，进一步证明了斑马鱼IL-17基因与其他生物IL-17基因的结构相似性。通过RT-PCR的表达分析显示IL-17基因在正常和刺激的肾、脾、鳃和肠组织中表达。IL-17在非刺激组织中的表达表明这些基因在正常条件下也可能发挥重要作用。(c)2006爱思唯尔有限公司保留所有权利。
Cytokines are one of the major signaling molecules involved in immunity. Many of these cytokines have been isolated in vertebrates and found to play a significant role in host defense mechanism. Interleukin-17 (IL-17) family of genes are known to have pro-inflammatory actions and associated with specific disease conditions, these genes are conserved across vertebrate evolution. In this study, computational screening for the zebrafish (Danio rerio) genome resulted in identification of five configs harboring IL-17 genes. Zebrafish cDNA encoding five IL-17 genes exhibited percentage identities of 19.3%-61.9% with that of human homologs. The molecules show conservation of cysteines, important for disulphide bonds for IL-17 molecules. The structural composition of these genes shows two introns and three exons except for IL-17D gene that has only one intron and two exons. Phylogenetic analysis using maximum parsimony algorithm showed that zebrafish IL-17 genes clustered well with other IL-17 homologs further proving the structural similarity with IL-17 genes from other organisms. Expression analysis by RT-PCR revealed expression of IL-17 genes in normal and stimulated tissues of kidney, spleen, gills and intestine. The expression of IL-17 in un-stimulated tissues indicates that these genes may play important roles in normal conditions as well. (c) 2006 Elsevier Ltd. All rights reserved.