lncRNA MNX1-AS1 Promotes Glioblastoma Progression Through Inhibition of miR-4443.
lncRNA MNX1-AS1 Promotes Glioblastoma Progression Through Inhibition of miR-4443.
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DOI：
10.3727/096504018x15228909735079
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发表时间：
2019-02-21
影响因子：
3.1
通讯作者：
Feng J
中科院分区：
文献类型：
--
作者：
Gao Y;Xu Y;Wang J;Yang X;Wen L;Feng J
长链非编码RNA（longnoncodingRNA，lncRNA）是人类多种肿瘤的重要调控因子。lncRNA MNX 1-AS 1已被证明是上皮性卵巢癌中的癌基因。然而，MNX 1-AS 1在胶质母细胞瘤（GBM）中的功能仍然很大程度上未知。我们发现MNX 1-AS 1在GBM组织和细胞系中表达显著上调。MNX 1-AS 1基因的敲除可显著抑制GBM细胞的增殖、迁移和侵袭。在机制方面，我们发现MNX 1-AS 1可以与GBM细胞中的miR-4443结合。过表达miR-4443显著抑制MNX 1-AS 1的表达，反之亦然。此外，MNX 1-AS 1和miR-4443在GBM组织中的表达水平之间存在负相关。我们发现miR-4443的过表达抑制GBM细胞的增殖、迁移和侵袭。我们还发现，抑制miR-4443逆转了MNX 1-AS 1敲低对GBM细胞增殖、迁移和侵袭的影响。综上所述，我们发现MNX 1-AS 1通过抑制miR-4443促进GBM细胞的增殖、迁移和侵袭。
Long noncoding RNAs (lncRNAs) have been acknowledged as important regulators in various human cancers. lncRNA MNX1-AS1 has been shown to be an oncogene in epithelial ovarian cancer. However, the function of MNX1-AS1 in glioblastoma (GBM) remains largely unknown. Here we found that the expression of MNX1-AS1 was significantly upregulated in GBM tissues and cell lines. Knockdown of MNX1-AS1 significantly inhibited the proliferation, migration, and invasion of GBM cells. In terms of mechanism, we found that MNX1-AS1 could bind to miR-4443 in GBM cells. Overexpression of miR-4443 significantly inhibited the expression of MNX1-AS1 and vice versa. Moreover, there was an inverse correlation between the expression levels of MNX1-AS1 and miR-4443 in GBM tissues. We found that overexpression of miR-4443 inhibited the proliferation, migration, and invasion of GBM cells. We also showed that inhibition of miR-4443 reversed the effects of MNX1-AS1 knockdown on GBM cell proliferation, migration, and invasion. Taken together, we found that MNX1-AS1 promoted the proliferation, migration, and invasion of GBM cells through inhibiting miR-4443.