Up-regulation of histamine H1 receptors in an allergic rat nasal mucosa model
Up-regulation of histamine H1 receptors in an allergic rat nasal mucosa model
复制标题
过敏性大鼠鼻粘膜模型中组胺 H1 受体的上调
DOI：
10.1007/s00011-003-0304-7
复制
发表时间：
2004
影响因子：
6.7
通讯作者：
H. Fukui
中科院分区：
文献类型：
--
作者：
Y. Murata;A. Miyoshi;Y. Kitamura;N. Takeda;H. Fukui
组胺通过组胺H1受体亚型(H1Rs)诱导鼻部过敏症状。H1Rs的上调和下调取决于条件，这些机制被认为在调节组胺信号方面很重要。在此之前，我们建立了甲苯2，4-二异氰酸酯(TDI)致敏豚鼠的鼻腔超敏动物模型，观察了TDI[1-3]对鼻黏膜神经源性炎症的影响[1-3]。用实时定量逆转录聚合酶链式反应(RT-PCR)方法检测H1R基因的表达水平。我们还观察了d-氯苯那敏(CHP)和地塞米松(DEX)对TDI诱导的H1R基因表达和鼻部症状的影响。应用TDI可使H1R基因表达增加，而CHP和DEX可逆转这一变化。放射配基受体结合分析显示，H1R分子在TDI刺激下显著上调。
Histamine induces nasal allergy symptoms via histamine H1 receptor subtypes (H1Rs). H1Rs are up-and down-regulated depending on conditions, and the mechanisms are thought to be important in regulating histamine signaling. Previously, we developed a nasal hypersensitivity animal model involving guinea pigs sensitized to toluene 2, 4-diisocyanate (TDI), and observed neurogenic inflammation in the nasal mucosa on exposure to TDI [1–3].In this study, we examined both the expression levels of H1R mRNAs and molecules in the nasal mucosa of TDI sensitized rats. H1R mRNA levels were determined by the quantitative real-time reverse-transcriptase polymerase chain reaction (RT-PCR) method. We also investigated the effect of d-chlorpheniramine (CHP) and dexamethasone (DEX) on TDI-induced H1R mRNA expression and nasal symptoms. Application of TDI led to an increase in H1R mRNA levels and pretreatment with CHP and DEX reversed the increase. Radioligand receptor binding assays revealed that H1R molecules were significantly up-regulated by TDI provocation.