Characterization of a carboxypeptidase A gene from the mosquito, Aedes aegypti
Characterization of a carboxypeptidase A gene from the mosquito, Aedes aegypti
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DOI：
10.1046/j.1365-2583.2000.00159.x
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发表时间：
2000-02-01
影响因子：
2.6
通讯作者：
Jacobs-Lorena, M
中科院分区：
文献类型：
--
作者：
Edwards, MJ;Moskalyk, LA;Jacobs-Lorena, M
克隆了埃及伊蚊肠道特异性羧肽酶A基因（AeCPA）。该基因有一个开放的阅读框架，预测427个氨基酸的蛋白质，其中61%是相同的冈比亚按蚊羧肽酶A序列。AeCPA mRNA在幼虫和蛹发育过程中均未检测到。原位杂交实验表明，AeCPA mRNA表达的后中肠上皮细胞。与安形成鲜明对比。冈比亚羧肽酶A基因的表达，AeCPA mRNA积累到高水平，只有后期（约16-24小时后，摄入血餐。AeCPA基因诱导的时间分布与Ae相似。埃及晚期胰蛋白酶，这表明存在共同的调节元件。
A gut-specific carboxypeptidase A gene (AeCPA) from the mosquito, Aedes aegypti, was cloned and characterized. The gene has an open reading frame that predicts a protein of 427 amino acids, 61% of which are identical to an Anopheles gambiae carboxypeptidase A sequence. AeCPA messenger RNA (mRNA) was not detected during larval and pupal development. In situ hybridization experiments indicated that AeCPA mRNA is expressed by posterior midgut epithelial cells. In sharp contrast to An. gambiae carboxypeptidase A gene expression, AeCPA mRNA accumulates to high levels only late (approximate to 16-24 h) after ingestion of a blood meal. The temporal profile of AeCPA gene induction is similar to that of Ae. aegypti late trypsin, suggesting the existence of common regulatory elements.