Effect of eliminase gene (elmA) deletion on heparosan production and shedding in Escherichia coli K5.
Effect of eliminase gene (elmA) deletion on heparosan production and shedding in Escherichia coli K5.
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DOI：
10.1016/j.jbiotec.2013.03.018
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发表时间：
2013-06-10
影响因子：
4.1
通讯作者：
Dordick, Jonathan S.
中科院分区：
文献类型：
--
作者：
Hickey, Anne Marie;Bhaskar, Ujjwal;Linhardt, Robert J.;Dordick, Jonathan S.
E.大肠杆菌K5产生肝素原，并以温度依赖性方式将其释放到生长培养基中。脱落被认为是控制，至少部分地，通过酶作用于细胞相关的荚膜多糖，heparosan。这种脱落中的一种候选酶是消除酶。将elmA基因从大肠杆菌基因组中删除。coli K5中表达，并研究其对分泌型和细胞结合型肝素原的影响。消除酶基因的缺失导致肝素前体向培养基中的脱落和细胞囊中肝素前体含量的显著减少，表明其在E. coli K5。
E. coli K5 produces heparosan and sheds it into the growth medium in a temperature dependent manner. The shedding is believed to be controlled, at least in part, by enzyme action on the cell-associated capsular polysaccharide, heparosan. One candidate enzyme in such shedding is eliminase. The eliminase gene (elmA) was deleted from the genome of E. coli K5 and its effect on secreted and cell-associated heparosan was investigated. Deletion of the eliminase gene resulted in a significant reduction in heparosan shedding into the medium and heparosan content in the capsule of the cells, indicating its pivotal role in heparosan synthesis and shedding by E. coli K5.
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影响因子：
3.8
作者：
Wang, Zhenyu;Ly, Mellisa;Zhang, Fuming;Zhong, Weihong;Suen, Amy;Hickey, Anne Marie;Dordick, Jonathan S.;Linhardt, Robert J.
通讯作者：
Linhardt, Robert J.
影响因子：
3.2
作者：
Clarke, BR;Esumeh, F;Roberts, IS
通讯作者：
Roberts, IS
影响因子：
4.8
作者：
Xu, Yongmei;Pempe, Elizabeth H.;Liu, Jian
通讯作者：
Liu, Jian
影响因子：
3.2
作者：
Zorraquino, Violeta;Garcia, Begona;Solano, Cristina
通讯作者：
Solano, Cristina
影响因子：
3.2
作者：
Legoux, R;Lelong, P;Salome, M
通讯作者：
Salome, M