PlexinA3 restricts spinal exit points and branching of trunk motor nerves in embryonic zebrafish
PlexinA3 restricts spinal exit points and branching of trunk motor nerves in embryonic zebrafish
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DOI：
10.1523/jneurosci.1132-07.2007
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发表时间：
2007-05-02
影响因子：
5.3
通讯作者：
Becker, Catherina G.
中科院分区：
文献类型：
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作者：
Feldner, Julia;Reimer, Michell M.;Becker, Catherina G.
斑马鱼躯干中的先驱初级运动轴突由多个线索沿着它们的通路引导。丛状蛋白是影响运动轴突生长和路径发现的信号蛋白的受体成分。我们克隆了plexinA3在斑马鱼和本地化plexinA3 mRNA在初级运动神经元轴突生长。反义吗啉敲低导致运动轴突生长的实质性错误。错误包括初级运动神经的异常分支以及脊髓轴突的额外出口点。在运动轴突的腹侧和背侧通路上均发现了额外的分支和额外的神经。躯干环境和其他几种类型的轴突，包括三叉神经轴突，并没有受到plexinA3敲低的影响。RNA过表达挽救了所有吗啉代效应。联合吗啉注射的协同作用表明丛蛋白A3与脑信号蛋白3A同源物的相互作用。因此，丛蛋白A3是初级运动神经元中轴突引导线索的关键受体。
The pioneering primary motor axons in the zebrafish trunk are guided by multiple cues along their pathways. Plexins are receptor components for semaphorins that influence motor axon growth and path finding. We cloned plexinA3 in zebrafish and localized plexinA3 mRNA in primary motor neurons during axon outgrowth. Antisense morpholino knock-down led to substantial errors in motor axon growth. Errors comprised aberrant branching of primary motor nerves as well as additional exit points of axons from the spinal cord. Excessively branched and supernumerary nerves were found in both ventral and dorsal pathways of motor axons. The trunk environment and several other types of axons, including trigeminal axons, were not detectably affected by plexinA3 knock-down. RNA overexpression rescued all morpholino effects. Synergistic effects of combined morpholino injections indicate interactions of plexinA3 with semaphorin3A homologs. Thus, plexinA3 is a crucial receptor for axon guidance cues in primary motor neurons.