THE NEAR-WALL EXCESS OF PLATELET-SIZED PARTICLES IN BLOOD-FLOW - ITS DEPENDENCE ON HEMATOCRIT AND WALL SHEAR RATE
THE NEAR-WALL EXCESS OF PLATELET-SIZED PARTICLES IN BLOOD-FLOW - ITS DEPENDENCE ON HEMATOCRIT AND WALL SHEAR RATE
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DOI：
10.1016/0026-2862(87)90018-5
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发表时间：
1987-03-01
影响因子：
3.1
通讯作者：
ECKSTEIN, EC
中科院分区：
文献类型：
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作者：
TILLES, AW;ECKSTEIN, EC
方法涉及显微镜，以获得浓度分布的血小板大小珠在流动过程中通过玻璃通道。荧光乳胶珠悬浮液（2.38 .mu）用等渗白蛋白-葡萄糖溶液制备红细胞。注射泵调节悬浮液通过50或100 .mu的玻璃通道的流量。米宽;大多数实验采用的剪力墙速率为1630秒-1。通过频闪荧光显微镜，在平行于通道壁的图像平面上收集照片。通过对照片中聚焦的微球图像进行计数，得出微球在窄通道方向上的浓度分布图。结果表明，当悬浮液中含有显著比例的红细胞（超过7%）时，微珠的近壁过量发生。在血细胞比容为15%至45%（研究中最高）的情况下，过量浓度是中部地区浓度的5倍以上。对两种宽度的孔道进行试验，结果表明，孔道的多余面积为5 ~ 8 μ m。米厚。50亩的一系列实验。当悬浮红细胞比容为15%，壁面剪切速率为50 ~ 3180秒-1时，M通道的近壁运动仅在壁面剪切速率为430秒-1及以上时才较大。这项工作证明了壁面剪切速率（流速）和红细胞压积对表面附近血小板大小的珠粒数量的影响，从而说明了在血液表面相互作用中起作用的物理（流变学）因素。
Methods involving microscopy were used to obtain concentration profiles of platelet-sized beads during flow through glass channels. Suspensions of fluorescent latex beads (2.38 .mu.m diam) and washed red blood cells were made from an isotonic albumin-dextrose solution. A syringe pump regulated the suspension flow through glass channels, which were either 50 or 100 .mu.m wide; most experiments used a wall shear rate of 1630 sec-1. Via stroboscopic epifluorescence microscopy, photographs were collected on image planes parallel to the channel wall. Profiles of the bead concentration in the narrow channel direction were made by assembling counts of the focused bead images in the photographs. The results showed that a near-wall excess of the beads occurred when the suspension contained a significant fraction of red cells (over 7%). For hematocrits of 15 to 45% (the highest studied), the excess was above five times the concentration in the central region. Experiments with channels of both widths showed the region of excess beads was 5 to 8 .mu.m thick. A series of experiments with 50-.mu.m channels, with a suspension hematocrit of 15%, and with wall shear rates from 50 to 3180 sec-1 showed that near-wall exercises were large only for wall shear rates of 430 sec-1 and above. This work demonstrated the effects of wall shear rate (flow rate) and hematocrit on the number of platelet-sized beads near a surface and hence illustrated physical (rheological) factors that act in blood-surface interaction.