Characterization of the decision network for wing expansion in Drosophila using targeted expression of the TRPM8 channel.
Characterization of the decision network for wing expansion in Drosophila using targeted expression of the TRPM8 channel.
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DOI：
10.1523/jneurosci.4241-08.2009
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发表时间：
2009-03-18
期刊：
影响因子：
--
通讯作者：
White BH
中科院分区：
文献类型：
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作者：
Peabody NC;Pohl JB;Diao F;Vreede AP;Sandstrom DJ;Wang H;Zelensky PK;White BH
羽化后，成年苍蝇和其他昆虫选择合适的栖息地并展开翅膀。翅膀的膨胀是由激素bursicon控制的，在不利的环境条件下会延迟。环境因素如何延迟囊子释放和改变鲈鱼的选择和扩张行为还没有详细研究。在这里，我们提供的证据表明，在果蝇的运动程序的鲈鱼选择和翅膀扩张有不同的环境依赖性。使用物理操作，我们表明，栖息的决定主要是基于环境的估值和增量延迟的条件下，增加扰动和限制。与此相反，全或无电机模式背后的翅膀扩张是相对不变的长度，无论环境条件。使用一种新的技术，有针对性地激活神经元，我们表明，高度刻板的翅膀扩张运动模式可以启动刺激的NCCAP，一个小的网络的中央神经元，调节bursicon的释放。使用冷敏感大鼠TRPM8通道激活该网络足以触发翅膀扩展所需的所有基本行为和躯体过程。因此，在不利情况下机翼展开的延迟将环境敏感的决策网络耦合到启动固定动作模式的命令式网络。由于NCCAP介导的环境不敏感蜕皮相关的行为在果蝇的发展之前，成年出现，翅膀扩张的研究承诺的见解，不仅网络如何介导的行为选择，但也到决策网络如何发展。
After emergence, adult flies and other insects select a suitable perch and expand their wings. Wing expansion is governed by the hormone bursicon and can be delayed under adverse environmental conditions. How environmental factors delay bursicon release and alter perch selection and expansion behaviors has not been investigated in detail. Here we provide evidence that in Drosophila the motor programs underlying perch selection and wing expansion have different environmental dependencies. Using physical manipulations, we demonstrate that the decision to perch is based primarily on environmental valuations and is incrementally delayed under conditions of increasing perturbation and confinement. In contrast, the all-or-none motor patterns underlying wing expansion are relatively invariant in length regardless of environmental conditions. Using a novel technique for targeted activation of neurons, we show that the highly stereotyped wing expansion motor patterns can be initiated by stimulation of NCCAP, a small network of central neurons that regulates the release of bursicon. Activation of this network using the cold-sensitive rat TRPM8 channel is sufficient to trigger all essential behavioral and somatic processes required for wing expansion. The delay of wing expansion under adverse circumstances thus couples an environmentally-sensitive decision network to a command-like network that initiates a fixed action pattern. Because NCCAP mediates environmentally-insensitive ecdysis-related behaviors in Drosophila development prior to adult emergence, the study of wing expansion promises insights not only into how networks mediate behavioral choices, but also into how decision networks develop.