PCR-based Approaches for the Detection of Clinical Methicillin-resistant Staphylococcus aureus.
PCR-based Approaches for the Detection of Clinical Methicillin-resistant Staphylococcus aureus.
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DOI：
10.2174/1874285801610010045
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Ji Y
中科院分区：
文献类型：
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作者：
Liu Y;Zhang J;Ji Y
金黄色葡萄球菌是一种重要的病原体，可引起人和动物的各种感染，包括浅表和系统感染。由于治疗MRSA感染的选择有限，耐多药金黄色葡萄球菌，特别是耐甲氧西林金黄色葡萄球菌的持续出现，已经造成了巨大的经济负担和公共卫生方面的担忧。为了正确选择治疗方案，了解耐甲氧西林金黄色葡萄球菌(MRSA)的流行情况，准确、及时地诊断引起患者特定感染的病原体，揭示病原菌的耐药谱是至关重要的。在这篇综述中，我们概述了已成功用于快速检测金黄色葡萄球菌的不同方法，包括直接从各种临床标本中提取的MRSA和MSSA。指出了检测的敏感性和特异性。讨论了上市方法的优点和缺点。重要的是，需要另一种方法来进一步确认分子诊断分析的检测结果。
Staphylococcus aureus is an important pathogen that can cause a variety of infections, including superficial and systematic infections, in humans and animals. The persistent emergence of multidrug resistant S. aureus, particularly methicillin-resistant S. aureus, has caused dramatically economic burden and concerns in the public health due to limited options of treatment of MRSA infections. In order to make a correct choice of treatment for physicians and understand the prevalence of MRSA, it is extremely critical to precisely and timely diagnose the pathogen that induces a specific infection of patients and to reveal the antibiotic resistant profile of the pathogen. In this review, we outlined different PCR-based approaches that have been successfully utilized for the rapid detection of S. aureus, including MRSA and MSSA, directly from various clinical specimens. The sensitivity and specificity of detections were pointed out. Both advantages and disadvantages of listed approaches were discussed. Importantly, an alternative approach is necessary to further confirm the detection results from the molecular diagnostic assays.