ANAEROBIOSIS, FORMATE, NITRATE, AND PYRA ARE INVOLVED IN THE REGULATION OF FORMATE HYDROGENLYASE IN SALMONELLA-TYPHIMURIUM
ANAEROBIOSIS, FORMATE, NITRATE, AND PYRA ARE INVOLVED IN THE REGULATION OF FORMATE HYDROGENLYASE IN SALMONELLA-TYPHIMURIUM
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DOI：
10.1128/jb.158.3.972-977.1984
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发表时间：
1984-01-01
影响因子：
3.2
通讯作者：
MACY, J
中科院分区：
文献类型：
--
作者：
BARRETT, EL;KWAN, HS;MACY, J
从S.用噬菌体Mu d（Apr lac）进行转座诱变。一组由缺乏氢化酶的菌株组成。该组的突变位点位于已知hyd基因附近。第二组由缺乏与氢化酶相关的甲酸脱氢酶的突变体组成。其中4例定位到突变位点。它不在先前描述的fhlD基因附近，而是位于沙门氏菌图谱上的93 min处。第3突变群，其中包括菌株，产生气体的三糖铁琼脂，但不是在营养琼脂补充葡萄糖，似乎是皮拉突变体。插入位点位于皮拉附近，它们需要精氨酸和嘧啶才能生长。在hyd突变体中lac操纵子的表达由厌氧诱导。在厌氧条件下添加甲酸盐仅使其略有增加，而添加硝酸盐则使其略有降低。硝酸盐在也携带chlC：：Tn10插入的hyd：：Mu d菌株中没有影响。乳糖操纵子在fhl突变体中的完全表达需要甲酸盐和厌氧条件。硝酸盐的存在下，除了甲酸导致的活动约一半，在其不存在下，即使在fhl：：Mu d chlC：：Tn10双突变体。在不存在甲酸盐的情况下，硝酸盐仅在fhl：：Mu d单突变体中降低表达。皮拉突变体中lac操纵子的表达受到精氨酸和胞嘧啶以及缺氧的抑制。皮拉参与有氧和无氧代谢的解释提出。
Three groups of mutants defective in the fermentative production of gas were isolated from S. typhimurium LT2 subjected to transposition mutagenesis with phage Mu d(Apr lac). One group consisted of strains which lacked hydrogenase. The mutation site for this group was located in the vicinity of the known hyd gene. A 2nd group consisted of mutants which lacked the formate dehydrogenase associated with hydrogenase. The mutation site was located in 4 of them. It was not in the vicinity of the previously described fhlD gene but was instead located at 93 min on the Salmonella map. The 3rd mutant group, which consisted of strains that produced gas in triple sugar iron agar but not in nutrient agar supplemented with glucose, appeared to be pyrA mutants. The insertion site was located in the vicinity of pyrA, and they required arginine and pyrimidines for growth. Expression of the lac operon in the hyd mutants was induced by anaerobiosis. It was only slightly increased by the addition of formate under anaerobic conditions and slightly decreased by the addition of nitrate. Nitrate had no effect in an hyd::Mu d strain that also carried a chlC::Tn10 insertion. Full expression of the lac operon in the fhl mutants required both formate and anaerobic conditions. The presence of nitrate in addition to formate resulted in activities about half those obtained in its absence, even in the fhl::Mu d chlC::Tn10 double mutant. In the absence of formate, nitrate reduced expression only in the fhl::Mu d single mutants. Expression of the lac operon among the pyrA mutants was repressed by arginine and cytosine and also by anaerobiosis. An explanation for the involvement of pyrA in aerobic and anaerobic metabolism is proposed.