Efficient protoplast isolation from ovule-derived embryogenic callus in Citrus volkameriana
Efficient protoplast isolation from ovule-derived embryogenic callus in Citrus volkameriana
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DOI：
10.3906/tar-1912-16
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发表时间：
2020-01-01
影响因子：
2.9
通讯作者：
Cimen, Berken
中科院分区：
文献类型：
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作者：
Cimen, Berken
本研究报告了从 Volkameriana (Citrus volkameriana L.) 胚芽衍生的胚性愈伤组织中分离有活力的原生质体，这是柠檬生产中需求量很大的砧木。在 3 个不同的未成熟果实阶段，包括花后 4、8 和 12 周 (WAA) 分离的 C. volkameriana 胚珠，在 5 种不同的培养基上培养，以产生胚性愈伤组织系作为柠檬生产的来源材料。原生质体分离。 EME培养基（MT基础培养基+0.5 gL(-1)麦芽提取物），添加不同浓度的植物激素[激动素（KIN）、2,4-二氯苯氧乙酸（2,4-D）和6-苄氨基嘌呤（BAP）]，进行愈伤组织测试。根据双向方差分析，未成熟果实阶段和培养基类型的结果确定了显着影响（P
The present study reports on the isolation of viable protoplast from ovule-derived embryogenic calli of Volkameriana (Citrus volkameriana L.), which is a rootstock in high demand for lemon production.Ovules of C. volkameriana isolated at 3 different immature fruit stages, comprising4, 8, and 12 weeks after anthesis (WAA), were cultured on5 different media in order to produce embryogenic callus lines as a source material for protoplast isolation. EME medium (MT basal medium + 0.5 gL(-1) malt extract), with the addition of phytohormones [kinetin (KIN), 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzylaminopurine (BAP)] at different concentrations, were tested for callogenesis. According to 2-way ANOVA, significant effects were determined as a result of the immature fruit stage and type of culture media (P