Structures of ribonucleoprotein particle modification enzymes.
Structures of ribonucleoprotein particle modification enzymes.
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DOI：
10.1017/s0033583510000235
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发表时间：
2011-02
影响因子：
6.1
通讯作者：
Li, Hong
中科院分区：
文献类型：
--
作者：
Liang, Bo;Li, Hong
小核仁和卡哈尔体核糖核蛋白颗粒（RNP）是核糖体和剪接体成熟所必需的。它们由小核仁RNA或Cajal体RNA与伴侣蛋白结合组成，代表最复杂的RNA修饰酶。结构和功能研究的最新进展揭示了有关核糖核蛋白组装和底物结合的详细信息。这些酶形成交织的RNA-蛋白质组装体，促进大核糖体RNA或小核RNA的可逆结合。这些发现解释了酶组装和底物修饰中组分之间的特异性。单个组件和完整RNP的多个构象表明动态组装过程，并证明体内需要许多组装因子。
Small nucleolar and Cajal body ribonucleoprotein particles (RNPs) are required for the maturation of ribosomes and spliceosomes. They consist of small nucleolar RNA or Cajal body RNA combined with partner proteins and represent the most complex RNA modification enzymes. Recent advances in structure and function studies have revealed detailed information regarding ribonucleoprotein assembly and substrate binding. These enzymes form intertwined RNA–protein assemblies that facilitate reversible binding of the large ribosomal RNA or small nuclear RNA. These revelations explain the specificity among the components in enzyme assembly and substrate modification. The multiple conformations of individual components and those of complete RNPs suggest a dynamic assembly process and justify the requirement of many assembly factors in vivo.