Synaptic vesicle proteins in cells of the sympathoadrenal lineage
Synaptic vesicle proteins in cells of the sympathoadrenal lineage
复制标题
DOI：
10.1016/s0165-1838(96)00100-2
复制
发表时间：
1996-12-14
期刊：
影响因子：
--
通讯作者：
Dahlstrom, A
中科院分区：
文献类型：
--
作者：
Hou, XE;Dahlstrom, A
交感肾上腺系细胞分化后表现出不同的特征，尽管它们在胚胎发育过程中来自同一个神经嵴前体。在本研究中，我们比较了几种突触囊泡蛋白在颈上神经节和肾上腺髓质的分布模式。利用间接免疫荧光联合共聚焦激光扫描显微镜观察到，抗整体突触囊泡膜蛋白（SV2、synaptotagmin I、synaptobrevin II和synaptophysin）的血清在这些细胞中引起强烈的免疫反应，而抗synaptobrevin I仅引起微弱的荧光。在细胞中还观察到突触囊泡相关蛋白Rab3a和SNAP25的免疫反应性。突触素- ia反应物质在染色质细胞中不存在，但在SCG和虹膜末梢的交感神经元中存在少量。另一方面，突触素IIa免疫反应物质在大多数SCG神经元和肾上腺素能虹膜终末强烈。神经特异性克拉拉蛋白轻链在SCG细胞和肾上腺神经节细胞中检测到，但在染色质细胞中仅观察到微弱的痕迹。在脑干儿茶酚胺神经元中表达的一种囊泡单胺递质VMAT2在SCG的大多数细胞中被观察到，在肾上腺髓质的细胞群中也观察到，其中VMAT2阳性的小染色质细胞是pnmt阴性的。SCG中的SIF细胞含有大多数所研究的突触囊泡蛋白。结果表明，交感神经元、SIF细胞和肾上腺染色质细胞分化后，尽管它们的生理结构不同，但仍具有许多相同的囊泡蛋白。
The cells of sympathoadrenal lineage display different characteristics after differentiation, although they stem from the same neural crest precursor during embryonic development. In the present study we compared the distribution patterns of several synaptic vesicle proteins in the superior cervical ganglion (SCG) and the adrenal medulla. Using indirect immunofluorescence combined with confocal laser scanning microscopy, it was observed that antisera against integral synaptic vesicle membrane proteins (SV2, synaptotagmin I, synaptobrevin II and synaptophysin) induced strong immunoreactivities in these cells, but anti-synaptobrevin I caused only a faint fluorescence. Immunoreactivities of the synaptic vesicle-associated proteins Rab3a and SNAP25 were also observed in the cells. Synapsin-Ia-reactive material appeared absent from chromaffin cells but present in small amounts in sympathetic neurons in the SCG and iris terminals. On the other hand, synapsin IIa immunoreactive material was strong in most SCG neurons and in adrenergic iris terminals. The neural specific clatrin light chain was detected in the SCG cells and in ganglion cells of the adrenal, but only weak traces could be observed in chromaffin cells. One of the vesicular monoamine transmitter transporters, VMAT2 which is expressed in catecholamine neurons in the brain stem, was observed in most cells in the SCG and also in groups of cells in the adrenal medulla, where the VMAT2-positive small chromaffin cells were PNMT-negative. SIF cells in the SCG contained most of the synaptic vesicle proteins investigated. The results show that after differentiation, sympathetic neurons, SIF cells and adrenal chromaffin cells still share many vesicle proteins even though their physiology is different.