Disruption of model cell membranes by carbon nanotubes
Disruption of model cell membranes by carbon nanotubes
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DOI：
10.1016/j.carbon.2013.03.057
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发表时间：
2013-08-01
期刊：
影响因子：
10.9
通讯作者：
Posner, Jonathan D.
中科院分区：
文献类型：
--
作者：
Corredor, Charlie;Hou, Wen-Che;Posner, Jonathan D.
碳纳米管（CNTs）是全球碳质工程纳米颗粒（ENPs）中产量最高的纳米材料之一，在生物医学、纳米复合材料和能源转换等领域具有潜在的应用前景。然而，碳纳米管可能的广泛使用以及相关的生物暴露可能性引发了人们对其纳米毒性和生态影响的担忧。在这项工作中，我们探测平面悬浮脂质双层膜，作为模型细胞膜，功能化的多壁碳纳米管（MWCNT），CdSe/ZnS量子点，和控制有机化合物，蜂毒肽，使用电生理测量平台的响应。电生理测量表明，在1.6-12 ppm的浓度范围内的多壁碳纳米管通过诱导显著的跨膜电流通量破坏脂质膜，这表明多壁碳纳米管插入并穿过脂质双层膜，形成允许离子运输的跨膜碳纳米管通道。本文演示了一个直接测量的离子迁移通过脂质双层诱导的碳纳米管。电生理测量可以提供独特的见解脂质双层-ENPs的相互作用，并有可能作为纳米毒性的初步筛选工具。(C)2013爱思唯尔有限公司保留所有权利。
Carbon nanotubes (CNTs) have one of the highest production volumes among carbonaceous engineered nanoparticles (ENPs) worldwide and are have potential uses in applications including biomedicine, nanocomposites, and energy conversion. However, CNTs possible widespread usage and associated likelihood for biological exposures have driven concerns regarding their nanotoxicity and ecological impact. In this work, we probe the responses of planar suspended lipid bilayer membranes, used as model cell membranes, to functionalized multi-walled carbon nanotubes (MWCNT), CdSe/ZnS quantum dots, and a control organic compound, melittin, using an electrophysiological measurement platform. The electrophysiological measurements show that MWCNTs in a concentration range of 1.6-12 ppm disrupt lipid membranes by inducing significant transmembrane current fluxes, which suggest that MWCNTs insert and traverse the lipid bilayer membrane, forming transmembrane carbon nanotubes channels that allow the transport of ions. This paper demonstrates a direct measurement of ion migration across lipid bilayers induced by CNTs. Electrophysiological measurements can provide unique insights into the lipid bilayer-ENPs interactions and have the potential to serve as a preliminary screening tool for nanotoxicity. (C) 2013 Elsevier Ltd. All rights reserved.