Effects of KAI gene expression on ferroptosis in pancreatic cancer cells
Effects of KAI gene expression on ferroptosis in pancreatic cancer cells
复制标题
KAI基因表达对胰腺癌细胞铁死亡的影响
DOI：
10.3892/mmr.2020.11802
复制
发表时间：
2021-02-01
影响因子：
3.4
通讯作者：
Han, Hao
中科院分区：
文献类型：
--
作者：
Liu, Xu;Guo, Xiaozhong;Han, Hao
铁下垂在胰腺癌（PC）发展中的具体作用和机制尚不清楚。本研究旨在探讨KAI1基因过表达对人PC细胞系MIA PaCa-2铁下垂的影响。转染pCMV-KAI1的MIA PaCa-2细胞经G418和KAI1蛋白筛选后，采用western blotting分析。将稳定表达KAI1基因的MIA PaCa-2细胞命名为MIA PaCa-2-KAI1。采用细胞计数试剂盒-8检测MIA PaCa-2和MIA PaCa-2- kai1细胞的增殖能力。流式细胞术比较细胞内活性氧（ROS）含量。western blotting检测转运铁蛋白（FPN）和谷胱甘肽过氧化物酶4 （GPX4）蛋白的表达。确认KAI1稳定表达细胞系，并将其重新标记为MIA PaCa-2-KAI1。MIA PaCa-2和MIA PaCa-2- kai1的增殖无显著差异。与MIA PaCa-2（从2.94 +/- 0.02到2.95 +/- 0.02）相比，MIA PaCa-2（从2.94 +/- 0.02到2.95 +/- 0.02）的增殖增加更为明显
The specific role and mechanism of ferroptosis in the development of pancreatic cancer (PC) remain to be elucidated. The present study aimed to investigate the effects of the overexpression of the KAI1 gene on the ferroptosis of the human PC cell line MIA PaCa-2. MIA PaCa-2 cells infected with pCMV-KAI1 and selected by G418 and KAI1 protein were analyzed by western blotting. The MIA PaCa-2 cells with a stable expression of the KAI1 gene were termed MIA PaCa-2-KAI1. The proliferative capacities of MIA PaCa-2 and MIA PaCa-2-KAI1 cells were detected using Cell Counting Kit-8. The reactive oxygen species (ROS) in the cells were compared by flow cytometry. The expressions of ferroportin (FPN) and glutathione peroxidase 4 (GPX4) protein were analyzed by western blotting. The KAI1 stable expression cell line was confirmed and relabeled as MIA PaCa-2-KAI1. No significant differences in the proliferation of MIA PaCa-2 and MIA PaCa-2-KAI1 were identified. Following treatment with a ferroptosis blocker, the increase in the proliferation of MIA PaCa-2-KAI1 (from 2.06 +/- 0.02 to 2.75 +/- 0.02) was more evident compared with MIA PaCa-2 (from 2.94 +/- 0.02 to 2.95 +/- 0.02; P