Preferential survival of CD8 T and NK cells expressing high levels of CD94
Preferential survival of CD8 T and NK cells expressing high levels of CD94
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DOI：
10.4049/jimmunol.170.4.1737
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发表时间：
2003-02-15
影响因子：
4.4
通讯作者：
Forman, J
中科院分区：
文献类型：
--
作者：
Gunturi, A;Berg, RE;Forman, J
Qa-1(B)/Qdm四聚体与CD94/NKG2受体结合，高水平表达在50%的小鼠NK细胞上。虽然来自幼鼠的CD8 T细胞很少表达CD94/NKG2受体，但从对单核细胞增生性李斯特菌(Lm)发生二次反应的小鼠中提取的CD8 T细胞中，有50%是CD94(高)，并与四聚体结合。虽然CD94(Int)NK细胞不与四聚体结合，但CD94(Int)CD8T细胞与四聚体结合，这种结合依赖于CD8辅助受体。我们发现CD8、T和NK细胞的凋亡程度与CD94的表达呈负相关，培养1-3天后CD94(高)细胞的凋亡率较低。CD8T细胞存活率的差异在培养后6h就明显存在，并持续到48h几乎所有的CD94(neg/int)细胞都发生了凋亡。相反，抑制性Ly-49A、G2、C/I分子的表达与较高的细胞凋亡率相关。交联型CD94/NKG2受体可进一步降低表达CD94的小鼠CD8T细胞的凋亡率，而交联型Ly-49I对表达Ly-49I的CD8T细胞无影响。交联化CD8 T细胞上的CD3增加了继发性LM感染小鼠的凋亡程度，但同时交联CD94/NKG2受体阻止了这一过程。在NK细胞中也观察到了类似的结果，CD94(高)细胞在培养1-3天后显示出比CD94(INT)细胞更少的凋亡。因此，CD94/NKG2的表达与较低的细胞凋亡水平相关，可能在CD8 T细胞和NK细胞的维持中起重要作用。《免疫学杂志》，2003年。
The Qa-1(b)/Qdm tetramer binds to CD94/NKG2 receptors expressed at high levels on similar to50% of murine NK cells. Although very few CD8 T cells from naive mice express CD94/NKG2 receptors, similar to50% of CD8 T cells taken from mice undergoing a secondary response against Listeria monocytogenes (LM) are CD94(high) and bind the tetramer. Although CD94(int) NK cells do not bind the tetramer, CD94(int) CD8 T cells do, and this binding is dependent on the CD8 coreceptor. We found that the extent of apoptosis in CD8 T and NK cells was inversely related to the expression of CD94, with lower levels of apoptosis seen in CD94(high) cells after 1-3 days of culture. The difference in CD8 T cell survival was evident as early as 6 h after culture and persisted until nearly all the CD94(neg/int) cells were apoptotic by 48 h. In contrast, expression of inhibitory Ly-49A,G2,C/I molecules was associated with higher levels of apoptosis. Cross-linking CD94/NKG2 receptors on CD8 T cells from a mouse undergoing an LM infection further reduced the percentage of apoptotic cells on the CD94-expressing populations, while cross-linking Ly-49I had no effect on CD8 T cells expressing Ly-49I. Cross-linking CD3 on CD8 T cells from a mouse undergoing a secondary LM infection increases the extent of apoptosis, but this is prevented by cross-linking CD94/NKG2 receptors at the same time. Similar results were observed with NK cells in that the CD94(high) population displayed less apoptosis than CD94(int) cells after 1-3 days in culture. Therefore, the expression of CD94/NKG2 is correlated with a lower level of apoptosis and may play an important role in the maintenance of CD8 T and NK cells. The Journal of Immunology, 2003.