Inhibition of glutamine synthetase in a549 cells during hyperoxia.
Inhibition of glutamine synthetase in a549 cells during hyperoxia.
复制标题
高氧期间 a549 细胞中谷氨酰胺合成酶的抑制。
DOI：
10.1165/ajrcmb.27.1.4664
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Stahl,Jennifer
中科院分区：
文献类型：
--
作者：
McGrath-Morrow,SharonA;Stahl,Jennifer
High oxygen concentrations are used in the treatment of acute respiratory distress syndrome and hyaline membrane disease. Hyperoxia, however, can damage alveolar epithelial cells through the release of free oxygen radicals. Supplemental glutamine (Gln) has recently been shown to increase survival of A549 cells, a distal epithelial cell line, during hyperoxia . We found that supplemental Gln (Gln + ) is essential for cell growth in A549 cells. In room air, cells without supplemental Gln (Gln − ) survived with BCL-2 levels similar to those of Gln + cells, but cell growth was minimal. We also evaluated the role of glutamine synthetase (GS) in A549 cells during hyperoxia. L-methionine sulfoximine (MSO), an irreversible inhibitor of GS, was added to Gln + and Gln − cells. In hyperoxia, Gln − cells had greater survival then Gln − cells treated with MSO. Supplemental Gln could rescue cells in hyperoxia from the effect of MSO, suggesting that GS, through the endogenous synthesis of Gln, could attenuate hyperoxic cell injury. In hyperoxia, cells treated with 10-mM concentrations of Gln had increased survival compared with cells receiving 2–mM concentrations. The higher concentration of Gln, however, did not decrease the percentage of cells undergoing necrosis.
