Depletion of the Nb CORE receptor drastically improves agroinfiltration productivity in older Nicotiana benthamiana plants
Depletion of the Nb CORE receptor drastically improves agroinfiltration productivity in older Nicotiana benthamiana plants
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DOI：
10.1101/2023.01.18.517935
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发表时间：
2023-01
影响因子：
13.8
通讯作者：
Isobel Dodds;Changlong Chen;Pierre Buscaill;R. V. D. van der Hoorn
中科院分区：
文献类型：
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作者：
Isobel Dodds;Changlong Chen;Pierre Buscaill;R. V. D. van der Hoorn
本氏烟草（Nicotiana benthamiana）越来越多地用于瞬时基因表达以产生抗体、疫苗和其他药物蛋白质，但瞬时基因表达在完全发育的6- 8周龄植物中是低的。这种低基因表达被认为是由根癌农杆菌的冷休克蛋白（CSP）的感知引起的。CSP受体是有争议的，因为NbCSPR和NbCORE都声称感知CSP。在这里，我们证明了CSP感知在NbCORE沉默但NbCSPR不沉默的6周龄植物中被废除。重要的是，较老的NbCORE沉默植物支持农杆菌渗入后GFP荧光和蛋白质水平的急剧增加。NbCORE耗尽植物中瞬时蛋白质产量的急剧增加为分子农业提供了新的机会，其中具有较大生物量的较老植物现在可以用于有效的蛋白质表达。
Nicotiana benthamiana is increasingly used for transient gene expression to produce antibodies, vaccines, and other pharmaceutical proteins but transient gene expression is low in fully developed, 6-8wk old plants. This low gene expression is thought to be caused by the perception the cold shock protein (CSP) of Agrobacterium tumefaciens. The CSP receptor is contested because both NbCSPR and NbCORE have been claimed to perceive CSP. Here, we demonstrate that CSP perception is abolished in 6wk-old plants silenced for NbCORE but not NbCSPR. Importantly, older NbCORE-silenced plants support a drastically increased level of GFP fluorescence and protein upon agroinfiltration. The drastic increase in transient protein production in NbCORE depleted plants offers new opportunities for molecular farming, where older plants with larger biomass can now be used for efficient protein expression.