Parentage assignment and extra-group paternity in a cooperative breeder:: the Seychelles warbler (Acrocephalus sechellensis)
Parentage assignment and extra-group paternity in a cooperative breeder:: the Seychelles warbler (Acrocephalus sechellensis)
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DOI：
10.1046/j.0962-1083.2001.01355.x
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发表时间：
2001-09-01
影响因子：
4.9
通讯作者：
Burke, T
中科院分区：
文献类型：
--
作者：
Richardson, DS;Jury, FL;Burke, T
我们描述的开发和初步应用的半自动亲子鉴定系统在塞舌尔莺（Acrocephalus sechellensis）。该系统使用荧光标记引物的14个多态性微卫星位点在两个多重加载组的基因型有效地超过96%的莺种群在Cousin岛。当与cervus计划一起使用时，该系统提供了足够的权力来分配塞舌尔莺的母性和父权，尽管与其合作育种系统和相对较低的遗传变异水平相关的并发症。亲子关系分析表明，下属的“帮手”女性以及占主导地位的“主要”女性产卵在公共巢，表明塞舌尔莺有一个中间水平的女性生殖偏斜，在替代极端的帮手在巢和联合筑巢系统之间。44%的助手成功繁殖，占所有后代的15%。40%的年轻人是群体外的父亲。
We describe the development and initial application of a semiautomated parentage testing system in the Seychelles warbler (Acrocephalus sechellensis). This system used fluorescently labelled primers for 14 polymorphic microsatellite loci in two multiplex loading groups to genotype efficiently over 96% of the warbler population on Cousin island. When used in conjunction with the program cervus, this system provided sufficient power to assign maternity and paternity within the Seychelles warbler, despite the complications associated with its cooperative breeding system and a relatively low level of genetic variation. Parentage analyses showed that subordinate 'helper' females as well as the dominant 'primary' females laid eggs in communal nests, indicating that the Seychelles warbler has an intermediate level of female reproductive skew, in between the alternative extremes of helper-at-the-nest and joint nesting systems. Forty-four per cent of helpers bred successfully, accounting for 15% of all offspring. Forty per cent of young resulted from extra-group paternity.