Computer tracking of germ cells in the cycle of the seminiferous epithelium and prediction of changes in cycle duration in animals commonly used in reproductive biology and toxicology.
Computer tracking of germ cells in the cycle of the seminiferous epithelium and prediction of changes in cycle duration in animals commonly used in reproductive biology and toxicology.
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DOI：
10.1002/j.1939-4640.1992.tb00297.x
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发表时间：
1992-05
影响因子：
--
通讯作者：
Rex A. Hess;Paul Chen
中科院分区：
文献类型：
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作者：
Rex A. Hess;Paul Chen
编写了一个名为“阶段”的计算机程序，以帮助跟踪生殖细胞以及生精上皮周期中随时间向前和向后的阶段。该程序结合了大鼠、小鼠、仓鼠、豚鼠、狗、兔子、公牛、种马、公羊、野猪、鹌鹑、猴子和人的精子发生的基本动力学。该程序非常灵活，允许用户更改循环持续时间和每个阶段的频率。 Stages 是为在个人计算机上使用而编译的，并且可以免费在软盘上使用，Stages 是菜单驱动的，不需要编程知识。该程序使用已发表的睾丸毒性和维生素 A 阶段同步数据进行了测试。一般来说，预测的细胞类型与观察到的细胞类型相似；讨论了观测数据和预测数据之间的差异。当循环持续时间改变时，阶段同步的预测数据与观测数据一致。该计划将提高研究影响精子发生的因素的速度和准确性。通过使用阶段，可以预测毒性研究中的目标细胞类型并长期跟踪受影响的细胞。预测的细胞类型还可以指示在后续实验中对哪些细胞进行生化和形态学检查。该计划对于初学者学习细胞关联的复杂模式以及生精上皮周期中生殖细胞的进展也很有用。
A computer program called Stages was written to aid the tracking of germ cells and stages forward and backward through time in the cycle of the seminiferous epithelium. The program incorporates the basic kinetics of spermatogenesis in the rat, mouse, hamster, guinea pig, dog, rabbit, bull, stallion, ram, boar, quail, monkey, and man. The program is flexible and permits the user to alter the cycle duration time and the frequency of each stage. Compiled for use on personal computers and available on floppy disks free of charge, Stages is menu-driven and requires no knowledge of programming. The program was tested using published data of testicular toxicity and vitamin A synchronization of stages. In general, predicted cell types were similar to those observed; discrepancies between observed and predicted data are discussed. When cycle duration time was changed, predicted data for stage synchronization coincided with the observed data. This program will improve the speed and accuracy of studying factors that affect spermatogenesis. By using Stages, it is possible to predict the target cell types in a toxicity study and to track affected cells over long periods of time. Predicted cell types may also indicate which cells to examine biochemically as well as morphologically in subsequent experiments. The program will also be useful to beginning students learning the complex patterns of cellular associations and the progression of germ cells in the cycle of the seminiferous epithelium.