Energy driven cascade recognition for selective detection of nucleic acids with high discrimination factor at room temperature.
Energy driven cascade recognition for selective detection of nucleic acids with high discrimination factor at room temperature.
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DOI：
10.1016/j.bios.2015.12.093
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发表时间：
2016-05
影响因子：
12.6
通讯作者：
Zhang Zhang-Zhang;Jun Yu Li;Juan Yao;Ting Wang;Dan Yin;Yu Xiang;Zhongping Chen;G. Xie
中科院分区：
文献类型：
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作者：
Zhang Zhang-Zhang;Jun Yu Li;Juan Yao;Ting Wang;Dan Yin;Yu Xiang;Zhongping Chen;G. Xie
在本文中，我们展示了一种级联识别策略，用于在室温下检测具有高辨别因子的单链核酸。级联识别策略包含立足点介导的链置换反应和双立足点介导的双链置换反应，从而能够高度识别目标点突变。模型靶标的区分因子在45~109之间，介质为70。该策略均质、易操作、无酶、等温，可轻松适应高通量设备，无需设计复杂的仪器。
In this article, we demonstrated a cascade recognition strategy for the detection of single strand nucleic acid with high discrimination factor at room temperature. The cascade recognition strategy contains a toehold mediated strand displacement and a double-toehold mediated double strand displacement reaction, thus enable the high ability to discern point mutation of target. The discrimination factor of the model target is between 45 and 109, with the medium of 70. This strategy is homogeneous, easy operation, enzyme-free, isothermal, and can be easily adapted to high-throughput devices without the need of designing complicated instruments.