A gene fusion at a homeobox locus: Alterations in leaf shape and implications for morphological evolution
A gene fusion at a homeobox locus: Alterations in leaf shape and implications for morphological evolution
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DOI：
10.1105/tpc.9.8.1289
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发表时间：
1997-08-01
期刊：
影响因子：
11.6
通讯作者：
Sinha, N
中科院分区：
文献类型：
--
作者：
Chen, JJ;Janssen, BJ;Sinha, N
复叶存在于许多被子植物属中，并且被认为与单叶或单叶的精心制作根本不同。knotted 1-like homeobox（knox）基因被认为是调节植物发育的基因。当在同源或异源物种中过表达时，该基因家族可引起叶形态的变化，包括番茄中的过度叶复合。我们在这里描述的一个例子，一个自发产生的融合之间的基因编码的代谢酶和同源结构域蛋白。我们表明，融合的结果在过表达的同源结构域蛋白和形态的变化，近似的变化所造成的过度表达的花椰菜花叶病毒35 S启动子的控制下，在转基因植物的相同基因。外显子改组事件可以解释蛋白质的模块性。如果重组的外显子与改变的启动子相关，则可能导致基因表达模式的改变。我们的研究结果表明，这种性质的基因融合可以引起表达模式的变化，导致形态改变。我们认为，这种现象可能在形式的演变中发挥了作用。
Compound leaves are seen in many angiosperm genera and are thought to be either fundamentally different from simple leaves or elaborations of simple leaves. The knotted1-like homeobox (knox) genes are known to regulate plant development. When overexpressed in homologous or heterologous species, this family of genes can cause changes in leaf morphology, including excessive leaf compounding in tomato. We describe here an instance of a spontaneously arisen fusion between a gene encoding a metabolic enzyme and a homeodomain protein. We show that the fusion results in overexpression of the homeodomain protein and a change in morphology that approximates the changes caused by overexpression of the same gene under the control of the cauliflower mosaic virus 35S promoter in transgenic plants. Exon-shuffling events can account for the modularity of proteins. If the shuffled exons are associated with altered promoters, changes in gene expression patterns can result. Our results show that gene fusions of this nature can cause changes in expression patterns that lead to altered morphology. We suggest that such phenomena may have played a role in the evolution of form.