Molecular cloning, characterization and expression profiles of three estrogen receptors in protogynous hermaphroditic orange-spotted grouper (Epinephelus coioides)
Molecular cloning, characterization and expression profiles of three estrogen receptors in protogynous hermaphroditic orange-spotted grouper (Epinephelus coioides)
复制标题
斜带石斑鱼（Epinephelus coioides）三种雌激素受体的分子克隆、表征和表达谱
DOI：
10.1016/j.ygcen.2011.03.025
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发表时间：
2011-07-01
影响因子：
2.7
通讯作者：
Lin, Haoran
中科院分区：
文献类型：
--
作者：
Chen, Huapu;Zhang, Yong;Lin, Haoran
雌性激素通过雌性激素受体（Estrogen receptor, er）作为调节途径在脊椎动物生殖系统中起着重要作用。本研究从一种原生硬骨鱼——橙斑石斑鱼（Epinephelus coioides）中分离到三条全长的ERs cDNA序列，分别为gER α 2235 bp、gER β 1 1967 bp和gER β 2 2158 bp。系统发育和氨基酸比对分析表明，每个gER都聚集在相应的类群中，在功能域上表现出高度的进化保守性。RT-PCR结果显示，所有获得的组织中均有不同水平的ger表达。gER α在成熟卵巢中高表达，gER β 1主要在未成熟卵巢中表达，gER β 2在卵巢发育过程中变化较大。在17 α -甲基睾酮（MT）植入诱导的雌雄倒转过程中，gER α逐渐降低，gER β 1逐渐升高，gER β 2先降低后恢复。本研究推测ger在原雌石斑鱼雌性成熟和MT诱导的雌雄倒转过程中可能发挥的作用。(C) 2011爱思唯尔公司版权所有。
Estrogen plays key roles in vertebrate reproductive system via estrogen receptors (ERs) as mediating pathways. In the present study, three full-length ERs cDNA sequences were isolated from a protogynous teleost, the orange-spotted grouper (Epinephelus coioides), and were 2235 bp for gER alpha, 1967 bp for gER beta 1 and 2158 bp for gER beta 2, respectively. Phylogenetic and amino acid alignment analyses showed that each gER was clustered in the corresponding taxonomic groups of the perciformes and exhibited high evolutional conservation in functional domains. RT-PCR revealed that gERs expressed at different levels in all the obtained tissues. gER alpha highly expressed in mature ovaries, gER beta 1 mainly expressed in immature ovaries and gER beta 2 varied greatly during ovarian development. During female to male sex reversal induced by 17 alpha-methyltestosterone (MT) implantation, gER alpha decreased gradually, gER beta 1 increased gradually, and gER beta 2 decreased firstly and recovered subsequently in male stage. The present study speculated the potential roles of gERs during female maturation and female to male sex reversal induced by MT in the protogynous grouper E. coioides. (C) 2011 Elsevier Inc. All rights reserved.