Genome-Wide Analysis of Glycoside Hydrolase Family 1-glucosidase Genes in Brassica rapa and Their Potential Role in Pollen Development
Genome-Wide Analysis of Glycoside Hydrolase Family 1-glucosidase Genes in Brassica rapa and Their Potential Role in Pollen Development
复制标题
甘蓝糖苷水解酶家族 1 β-葡萄糖苷酶基因的全基因组分析及其在花粉发育中的潜在作用
DOI：
10.3390/ijms20071663
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发表时间：
2019-04-01
影响因子：
5.6
通讯作者：
Hur, Yoonkang
中科院分区：
文献类型：
--
作者：
Dong, Xiangshu;Jiang, Yuan;Hur, Yoonkang
糖苷水解酶家族1 (GH1) -葡萄糖苷酶（BGLUs）由大量基因编码，参与植物的许多发育过程和胁迫反应。由于它们在植物生长发育中的重要性，人们已经对模式植物（拟南芥和水稻）和玉米进行了全基因组分析，但对重要的蔬菜作物芸苔属植物却没有进行全基因组分析。在本研究中，我们系统分析了rapa BGLUs (BrBGLUs)，并证明了几个基因参与花粉发育。在芸苔属植物数据库中鉴定出64个brbglu，它们被锚定在10条染色体上，有10个串联重复。系统发育分析表明，64个基因被划分为10个亚群，每个亚群具有相对保守的内含子/外显子结构。与拟南芥BGLUs （AtBGLUs）聚类有助于鉴定几个重要的亚群，这些亚群与类黄酮代谢、硫代葡萄糖苷的产生、脱落酸（ABA）水平的调节以及其他防御相关化合物有关。至少有6个brbglu可能参与花粉发育。BrBGLU10/AtBGLU20的表达、共表达基因的分析以及对拟南芥敲落植株的检测均表明BrBGLU10/AtBGLU20在花粉发育中具有不可缺少的功能。本研究结果可为进一步了解-葡萄糖苷酶的功能和培育富含营养成分的芸苔属作物提供有价值的信息。
Glycoside hydrolase family 1 (GH1) -glucosidases (BGLUs) are encoded by a large number of genes, and are involved in many developmental processes and stress responses in plants. Due to their importance in plant growth and development, genome-wide analyses have been conducted in model plants (Arabidopsis and rice) and maize, but not in Brassica species, which are important vegetable crops. In this study, we systematically analyzed B. rapa BGLUs (BrBGLUs), and demonstrated the involvement of several genes in pollen development. Sixty-four BrBGLUs were identified in Brassica databases, which were anchored onto 10 chromosomes, with 10 tandem duplications. Phylogenetic analysis revealed that 64 genes were classified into 10 subgroups, and each subgroup had relatively conserved intron/exon structures. Clustering with Arabidopsis BGLUs (AtBGLUs) facilitated the identification of several important subgroups for flavonoid metabolism, the production of glucosinolates, the regulation of abscisic acid (ABA) levels, and other defense-related compounds. At least six BrBGLUs might be involved in pollen development. The expression of BrBGLU10/AtBGLU20, the analysis of co-expressed genes, and the examination of knocked down Arabidopsis plants strongly suggests that BrBGLU10/AtBGLU20 has an indispensable function in pollen development. The results that are obtained from this study may provide valuable information for the further understanding of -glucosidase function and Brassica breeding, for nutraceuticals-rich Brassica crops.