The Glut1 and Glut4 glucose transporters are differentially expressed during perinatal and postnatal erythropoiesis
The Glut1 and Glut4 glucose transporters are differentially expressed during perinatal and postnatal erythropoiesis
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DOI：
10.1182/blood-2008-05-159269
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发表时间：
2008-12-01
期刊：
影响因子：
20.3
通讯作者：
Taylor, Naomi
中科院分区：
文献类型：
--
作者：
Montel-Hagen, Amelie;Blanc, Lionel;Taylor, Naomi
葡萄糖是生物体的主要能量来源，其在脊椎动物中的运输是一种普遍保守的特性。在所有细胞谱系中，人类红细胞表达最高水平的 Glut1 葡萄糖转运蛋白，每个细胞有超过 200 000 个分子。然而，我们最近报道，红细胞 Glut1 表达是缺乏维生素 C 的哺乳动物物种的一个特定特征，仅包括高等灵长类动物、豚鼠和果蝠。在这里，我们表明，在所有其他测试的哺乳动物物种中，Glut1 在新生儿期的红细胞中短暂表达。 Glut1 在红细胞分化的成红细胞阶段上调，并且在出生时存在于绝大多数小鼠红细胞 (RBC) 上。但值得注意的是，在经历贫血诱导的红细胞生成的成年小鼠中，Glut1 并未被诱导，并且在这些条件下，一种独特的转运蛋白 Glut4 的上调导致了葡萄糖转运的增加。 Sp3和Sp1转录因子被认为可以调节Glut1转录，我们发现同时抑制Glut1和诱导Glut4与显着增加的Sp3/Sp1比率相关。因此，小鼠和人红细胞中的葡萄糖转运蛋白表达模式是不同的。在小鼠中，出生后从 Glut1 转变为 Glut4，并且 Glut4 在贫血条件下进一步上调。 （血。2008；112：4729-4738）
Glucose is a major source of energy for living organisms, and its transport in vertebrates is a universally conserved property. Of all cell lineages, human erythrocytes express the highest level of the Glut1 glucose transporter with more than 200 000 molecules per cell. However, we recently reported that erythrocyte Glut1 expression is a specific trait of vitamin C-deficient mammalian species, comprising only higher primates, guinea pigs, and fruit bats. Here, we show that in all other tested mammalian species, Glut1 was transiently expressed in erythrocytes during the neonatal period. Glut1 was up-regulated during the erythroblast stage of erythroid differentiation and was present on the vast majority of murine red blood cells (RBCs) at birth. Notably though, Glut1 was not induced in adult mice undergoing anemia-induced erythropoiesis, and under these conditions, the up-regulation of a distinct transporter, Glut4, was responsible for an increased glucose transport. Sp3 and Sp1 transcriptions factors have been proposed to regulate Glut1 transcription, and we find that the concomitant repression of Glut1 and induction of Glut4 was associated with a significantly augmented Sp3/Sp1 ratio. Glucose transporter expression patterns in mice and human erythrocytes are therefore distinct. In mice, there is a postnatal switch from Glut1 to Glut4, with Glut4 further up-regulated under anemic conditions. (Blood. 2008; 112: 4729-4738)