A model system for long-term serum-free suspension organ culture of human fetal tissues: experiments on digits and skin from multiple body regions
A model system for long-term serum-free suspension organ culture of human fetal tissues: experiments on digits and skin from multiple body regions
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DOI：
10.1007/s004410050907
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发表时间：
1997-10
影响因子：
3.6
通讯作者：
J. Zeltinger;K. Holbrook
中科院分区：
文献类型：
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作者：
J. Zeltinger;K. Holbrook
我们优化了之前用于体外研究人类胎儿皮肤的悬浮器官培养（SOC）系统。使用 11 种培养基培养胎儿手指和皮肤样本（估计孕龄 59-91 天），培养时间为 3 或 4 周。培养物补充有 0%–10% 小牛血清，并暴露于 5%–45% 的氧气气氛中。根据培养组织的形态学和组织学特性选择的最佳条件是：在具有 45% 氧气气氛的无血清环境中的 DMEM-F12 培养基。精致的 SOC 系统支持躯干、背部、头皮和指端皮肤的表皮和真皮的维护和/或持续发育，以及毛囊、指甲和腺体的发育。总体而言，与使用其他体外培养系统时观察到的情况相比，发育和分化更接近地模拟体内状态。介绍了小牛血清和氧气水平的影响，并讨论了操纵该系统环境以研究皮肤发育机制的潜力。
We have optimized a suspension organ culture (SOC) system previously used to study human fetal skin in vitro. Eleven types of media were used to culture fetal digits and skin samples (ranging from 59–91 days of estimated gestational age) for periods of 3 or 4 weeks. The cultures were supplemented with 0%–10% bovine calf serum and exposed to 5%–45% oxygen atmospheres. Optimal conditions, which were selected based upon the morphologic and histologic properties of the cultured tissues, were: DMEM-F12 medium in a serum-free environment with a 45% oxygen atmosphere. The refined SOC system supports the maintenance and/or continued development of the epidermis and dermis of trunk, back, scalp, and digital skin, developing hair follicles, nails, and glands. Overall, development and differentiation mimicked the in vivo state more closely than that observed when using other in vitro culture systems. The effects of bovine calf serum and oxygen levels are presented, and the potential for manipulating the environment of this system for studying mechanisms of cutaneous development is also discussed.