Nitric oxide-releasing chitosan nanoparticles alleviate the effects of salt stress in maize plants
Nitric oxide-releasing chitosan nanoparticles alleviate the effects of salt stress in maize plants
复制标题
DOI：
10.1016/j.niox.2016.09.010
复制
发表时间：
2016-12-30
影响因子：
3.9
通讯作者：
Seabra, Amedea B.
中科院分区：
文献类型：
--
作者：
Oliveira, Halley C.;Gomes, Bruna C. R.;Seabra, Amedea B.
一氧化氮（NO）是参与植物对各种非生物胁迫反应的信号分子。然而，NO供体在农业中的应用受到这些化合物的不稳定性的阻碍。尽管NO释放纳米粒子的生物医学目的和各种纳米材料开发的农用化学品的载体系统的成功使用，在植物中的NO交付的纳米载体的潜在应用尚未进行测试。在此，我们报告的合成和表征的壳聚糖纳米粒子（CS NPs）含有NO供体S-亚硝基巯基丁二酸（S-亚硝基MSA）。将这些释放NO的NP在减轻盐对玉米植物的有害影响方面的效率与未包封的NO供体的效率进行比较。通过离子凝胶法合成纳米粒子，并将NO供体前体巯基琥珀酸（MSA）包封到CS纳米粒子中（包封率为91.07%）。将MSA-CS NP的游离巯基亚硝化，得到S-亚硝基-MSA-CS NP（NO释放NP）。将S-亚硝基-MSA掺入CS NP中允许持续的NO释放。与游离S-亚硝基-MSA相比，用S-亚硝基-MSA-CS NP处理盐胁迫玉米植物导致更高的叶S-亚硝基硫醇含量。此外，S-亚硝基-MSA-CS NP在改善盐对光系统II活性、叶绿素含量和玉米植物生长的有害影响方面比游离NO供体更有效，因为纳米封装的S-亚硝基-MSA的保护作用在较低剂量下实现。总之，这些结果证明了S-亚硝基-MSA纳米包封在盐胁迫下增加玉米植物中NO生物活性的积极影响。(C)2016 Elsevier Inc. All rights reserved.
Nitric oxide (NO) is a signaling molecule involved in plant response to various abiotic stresses. However, the application of NO donors in agriculture is hampered by the instability of these compounds. Despite the successful uses of NO-releasing nanoparticles for biomedical purposes and the variety of nano materials developed as carrier systems of agrochemicals, the potential applications of nanocarriers for NO delivery in plants have not yet been tested. Herein, we report the synthesis and characterization of chitosan nanoparticles (CS NPs) containing the NO donor S-nitroso-mercaptosuccinic acid (S-nitrosoMSA). The efficiency of these NO-releasing NPs in mitigating the deleterious effects of salinity on maize plants was compared to that of the non-encapsulated NO donor. The NPs were synthesized through ionotropic gelation process, and mercaptosuccinic acid (MSA), the NO donor precursor, was encapsulated into CS NPs (91.07% encapsulation efficiency). Free thiol groups of MSA-CS NPs were nitrosated, leading to S-nitroso-MSA-CS NPs (NO-releasing NPs). The incorporation of S-nitroso-MSA into CS NPs allowed a sustained NO release. Treatments of salt-stressed maize plants with S-nitroso-MSA-CS NPs resulted in a higher leaf S-nitrosothiols content compared to that of free S-nitroso-MSA. Moreover, S-nitroso-MSA-CS NPs were more efficient than was the free NO donor in the amelioration of the deleterious effects of salinity in photosystem II activity, chlorophyll content and growth of maize plants because the protective action of the nanoencapsulated S-nitroso-MSA was achieved at lower dosages. Overall, these results demonstrate the positive impact of S-nitroso-MSA nanoencapsulation in increasing NO bioactivity in maize plants under salt stress. (C) 2016 Elsevier Inc. All rights reserved.