Antibodies against Sulphoquinovosyl-diacyl glycerol and their Reactions with Chloroplasts
Antibodies against Sulphoquinovosyl-diacyl glycerol and their Reactions with Chloroplasts
复制标题
磺基喹诺酰二酰基甘油抗体及其与叶绿体的反应
DOI：
10.1515/znb-1970-0413
复制
发表时间：
1970
期刊：
影响因子：
--
通讯作者：
R. Berzborn
中科院分区：
文献类型：
--
作者：
A. Radunz;R. Berzborn
利用免疫学技术，我们试图确定的定位和方向的二叶绿体类囊体中的硫脂。在用金鱼草叶绿体板层系统免疫家兔的过程中，获得了抗血清，其沉淀出硫脂乳剂。直到第二次静脉注射才检测到抗硫脂抗体。将纯化的墨角藻硫脂吸附在甲基化牛血清白蛋白上，免疫获得抗血清。这些抗血清和抗金鱼草片层系统的抗血清与来自广泛不同的植物物种的硫脂制剂反应。磺脂抗血清不与乳液的单或二半乳糖基-二酰基甘油，卵磷脂或阴离子磷脂磷脂酰甘油。在蔗糖梯度上分离的无渗透压休克的板层系统不直接被硫脂抗血清凝集，但在Coombs试验或Uhlenbruck后的混合抗原凝集中可以实现间接凝集。破碎的叶绿体，然而，直接凝集的硫脂抗血清，以及层状系统已被打乱，在法国新闻或超声波处理。从凝集和凝集抑制实验与由不同方法制备的层状系统，它的结论是决定性的基团的磺脂是可访问的叶绿体的层状系统中的抗体。但是，硫脂分子可能位于类囊体内。
Using immunological techniques we tried to determine the localization and orientation of the sulpholipid of diloroplasts in thylakoids. During immunization of rabbits with lamellar systems of chloroplasts of Antirrhinum majus antisera were obtained which precipitated sulpholipid emulsions. Antibodies against the sulpholipid were not detected until a second series of intravenous injections. By immunizing purified sulpholipid of Fucus serratus, adsorbed to methylated bovine serum albumin, sulpholipid antisera were acquired. These antisera and the antisera against lamellar systems of Antirrhinum majus reacted with preparations of sulpholipid from widely different plant species. The sulpholipid antisera did not react with emulsions of the mono- or digalactosyl-diacyl glycerol, of lecithin or the anionic phosphatide phosphatidyl glycerol. Lamellar systems isolated without osmotic shock on a sucrose gradient were not agglutinated by sulpholipid antisera directly, but an indirect agglutination could be achieved in a Coombs-test or in a mixed antigen agglutination after Uhlenbruck. Broken chloroplasts, however, were agglutinated directly by sulpholipid antisera, as well lamellar systems which had been disrupted in a French-press or by ultrasonic treatment. From agglutination and agglutination inhibition experiments with lamellar systems prepared by different methods it is concluded that the determinant groups of the sulpholipid are accessible for antibodies in the lamellar system of chloroplasts. But probably the sulpholipid molecules are located inside the thylakoids.