Intermediate filament proteins increase during chronic stimulation of skeletal muscle.
Intermediate filament proteins increase during chronic stimulation of skeletal muscle.
复制标题
在骨骼肌的慢性刺激过程中，中间丝蛋白会增加。
DOI：
10.1007/bf00130240
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发表时间：
1995
影响因子：
2.7
通讯作者：
Reiser,PJ
中科院分区：
文献类型：
--
作者：
Baldi,JC;Reiser,PJ
慢性低频电刺激兔快缩骨骼肌诱导增加水平的两个中间丝蛋白，结蛋白和波形蛋白，在前3周的刺激。这些增加发生在与α-辅肌动蛋白表达变化和Z盘宽度增加相同的时间过程中，但先于收缩蛋白的快到慢的变化，这已被其他人描述。结蛋白和波形蛋白水平在刺激的前2周期间增加，此时结蛋白的增加似乎达到平台，而波形蛋白在刺激的3周内继续显著增加。波形蛋白的绝对量在所有时间点都低于结蛋白，然而在刺激期间结蛋白和波形蛋白水平的增加强烈相关，表明这两种蛋白质在肌肉转化的初始阶段协同增加。我们认为，中间丝蛋白的表达，这与Z-盘结构的改变相一致的快速增加，可能表明强化的肌纤维的受力超微结构，以响应刺激引起的活动增加。波形蛋白和结蛋白表达水平升高的存在表明，成熟的骨骼肌在快速到缓慢的转化过程中向中间丝蛋白表达的发育程序恢复。
Chronic low-frequency electrical stimulation of rabbit fast-twitch skeletal muscle induces increased levels of two intermediate filament proteins, desmin and vimentin, during the first 3 weeks of stimulation. These increases occur over the same timecourse as reported shifts in α-actinin expression and increased Z-disc width, but precede the fast-to-slow shifts in contractile proteins, which have been described by others. Desmin and vimentin levels increase during the first 2 weeks of stimulation, at which time the increase in desmin appears to plateau while vimentin continues to increase significantly through 3 weeks of stimulation. Absolute amounts of vimentin are lower than desmin at all time points, however increases in desmin and vimentin levels are strongly correlated during the stimulation period, suggesting that the two proteins are coordinately increased during the initial phases of muscle transformation. We suggest that rapid increases in the expression of intermediate filament proteins, which coincide with alterations in Z-disc structure, may indicate a fortification of the force-bearing ultrastructure of the muscle fibre in response to the increased activity that is induced by stimulation. The presence of vimentin and elevated levels of desmin expression suggest that mature skeletal muscle reverts toward a developmental program of intermediate filament protein expression during fast-to-slow transformation.