Microarray Analysis of Gene Expression Changes in Neuroplastin 65-Knockout Mice: Implications for Abnormal Cognition and Emotional Disorders
Microarray Analysis of Gene Expression Changes in Neuroplastin 65-Knockout Mice: Implications for Abnormal Cognition and Emotional Disorders
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Neuroplastin 65 敲除小鼠基因表达变化的微阵列分析：对异常认知和情绪障碍的影响
DOI：
10.1007/s12264-018-0251-5
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发表时间：
2018-10-01
影响因子：
5.6
通讯作者：
Yuan, Qionglan
中科院分区：
文献类型：
--
作者：
Li, Huanhuan;Zeng, Jiujiang;Yuan, Qionglan
Neuroplastin 65 (Np65) 是一种免疫球蛋白超家族细胞粘附分子，参与突触形成和可塑性。我们最近的研究表明，Np65 敲除（KO）小鼠表现出异常的认知和情绪障碍。然而，其根本机制仍不清楚。在本研究中，我们通过微阵列分析在Np65-KO小鼠中发现了588个差异表达基因。 RT-PCR 分析还揭示了与发育和突触结构相关的基因表达的改变，例如 Cdh1、Htr3a 和 Kcnj9。此外，蛋白质印迹证明，Np65-KO 小鼠中 Wnt-3（一种参与发育的 Wnt 蛋白）的表达降低。令人惊讶的是，MRI 和 DAPI 染色显示 Np65-KO 小鼠的侧脑室体积显着减少。总之，这些发现表明 Np65 的消除会影响基因表达，这可能导致大脑发育异常。这些结果为 Np65 缺陷小鼠大脑功能改变的机制提供了线索。
Neuroplastin 65 (Np65) is an immunoglobulin superfamily cell adhesion molecule involved in synaptic formation and plasticity. Our recent study showed that Np65-knockout (KO) mice exhibit abnormal cognition and emotional disorders. However, the underlying mechanisms remain unclear. In this study, we found 588 differentially-expressed genes in Np65-KO mice by microarray analysis. RT-PCR analysis also revealed the altered expression of genes associated with development and synaptic structure, such as Cdh1, Htr3a, and Kcnj9. In addition, the expression of Wnt-3, a Wnt protein involved in development, was decreased in Np65-KO mice as evidenced by western blotting. Surprisingly, MRI and DAPI staining showed a significant reduction in the lateral ventricular volume of Np65-KO mice. Together, these findings suggest that ablation of Np65 influences gene expression, which may contribute to abnormal brain development. These results provide clues to the mechanisms underlying the altered brain functions of Np65-deficient mice.