A mouse gene encoding an oocyte antigen associated with autoimmune premature ovarian failure
A mouse gene encoding an oocyte antigen associated with autoimmune premature ovarian failure
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DOI：
10.1210/en.140.8.3720
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发表时间：
1999-08-01
期刊：
影响因子：
4.8
通讯作者：
Nelson, LM
中科院分区：
文献类型：
--
作者：
Tong, ZB;Nelson, LM
自身免疫性卵巢早衰会导致年轻女性出现更年期症状和不孕。在胸腺切除后自身免疫性卵巢早衰的小鼠中也出现了类似的症状。我们证明，这些小鼠产生了针对位于卵母细胞胞浆(卵浆)中的125 kDa蛋白的抗体。通过用自身免疫血清筛选小鼠卵巢互补DNA表达文库，我们鉴定了一个新的小鼠基因，其转录产物为3.75kb，其表达仅限于卵母细胞。最长的开放阅读框(3333bp)编码一种卵母细胞特异性蛋白，命名为OP1(卵浆特异性蛋白1)。该蛋白由1111个氨基酸组成，预测相对分子质量为125,502 Da。根据它的一级结构，它似乎是新的，没有暗示细胞质以外的定位的基序。来自卵巢自身免疫小鼠的免疫血清能够与重组OP1发生特异性反应，这增加了OP1作为抗原在小鼠自身免疫性卵巢早衰中发挥作用的可能性。
Autoimmune premature ovarian failure causes young women to develop menopausal symptoms and infertility. A similar syndrome appears in mice with postthymectomy autoimmune premature ovarian failure. We demonstrate that these mice develop antibodies against a 125-kDa protein located in the oocyte cytoplasm (ooplasm). By screening a mouse ovarian complementary DNA expression library with autoimmune serum, we have identified a novel mouse gene with a 3.75-kb ovarian transcript, the expression of which is restricted to the oocyte. The longest open reading frame (3333 bp) encodes an oocyte-specific protein, designated OP1 (ooplasm-specific protein 1). The protein is composed of 1111 amino acids with a predicted molecular mass of 125,502 Da. Based on its primary structure, it appears to be novel and has no motifs to suggest a localization other than in the cytoplasm. The ability of immune serum from mice with ovarian autoimmunity to react specifically with recombinant OP1 raises the possibility that OP1 as an antigen may play a role in murine autoimmune premature ovarian failure.