Migration of monocytes after intracerebral injection at entorhinal cortex lesion site
Migration of monocytes after intracerebral injection at entorhinal cortex lesion site
复制标题
DOI：
10.1189/jlb.0511241
复制
发表时间：
2012-07-01
影响因子：
5.5
通讯作者：
Glumm, Jana
中科院分区：
文献类型：
--
作者：
Kaminski, Miriam;Bechmann, Ingo;Glumm, Jana
脑组织内缺乏经典淋巴管使CNS的免疫监视复杂化，因此，细胞从CNS实质中移出需要替代途径。尽管已经证明了血液来源的单核细胞的侵袭及其在完整BBB的轴突变性区域中转化为分枝的小胶质细胞样细胞，但仍然不清楚这些细胞是否永久驻留，经历凋亡，或离开大脑在淋巴器官中呈递抗原。使用小鼠ECL和注射表达GFP的单核细胞，我们跟踪了脾和LN中注射细胞的外观以及全头组织切片中的迁移途径。单核细胞从病变部位迁移至深部CLN，在第7天达到数量峰值，但在病变/注射后第21天，脾脏、浅表CLN和腹股沟LN中几乎不存在单核细胞。在整个头部切片中，发现GFP单核细胞附着于嗅神经，并在48 hpi位于鼻粘膜内。因此，单核细胞能够从病变的脑区迁移到深层CLN，并使用筛板作为出口途径。J. Leukoc. 92：31-39; 2012.
The lack of classical lymph vessels within brain tissue complicates immune surveillance of the CNS, and therefore, cellular emigration out of the CNS parenchyma requires alternate pathways. Whereas invasion of blood-derived mononuclear cells and their transformation into ramified, microglia-like cells in areas of axonal degeneration across an intact BBB have been demonstrated, it still remained unclear whether these cells reside permanently, undergo apoptosis, or leave the brain to present antigen in lymphoid organs. With the use of ECL of mice and injection of GFP-expressing monocytes, we followed the appearance of injected cells in spleen and LNs and the migratory pathways in whole-head histological sections. Monocytes migrated from the lesion site to deep CLNs, peaking in number at Day 7, but they were virtually absent in spleen and in superficial CLNs and inguinal LNs until Day 21 after lesion/injection. In whole-head sections, GFP monocytes were found attached to the olfactory nerves and located within the nasal mucosa at 48 hpi. Thus, monocytes are capable of migrating from lesioned brain areas to deep CLNs and use the cribriform plate as an exit route. J. Leukoc. Biol. 92: 31-39; 2012.