Purinergic receptor distribution in endothelial cells in blood vessels: a basis for selection of coronary artery grafts
Purinergic receptor distribution in endothelial cells in blood vessels: a basis for selection of coronary artery grafts
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DOI：
10.1016/s0021-9150(01)00681-5
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发表时间：
2002-05-01
期刊：
影响因子：
5.3
通讯作者：
Barden, JA
中科院分区：
文献类型：
--
作者：
Ray, FR;Huang, W;Barden, JA
在乳内动脉内皮细胞层中检测嘌呤能P2X受体亚单位（P2X（1）至P2X（7））和P2Y（2）的表达水平（Ann.Thorac. 54（1992）652）、桡动脉（Ann.Thorac. 16（1973）111）和隐静脉（Ann.Thorac. Surg.20（1975）628）使用免疫组织化学和共聚焦显微镜在冠状动脉旁路移植手术中获得的样品。P2X（1）、P2X（2）、P2X（3）、P2X（7）和P2Y（2）在所有检查的血管中的内皮细胞中发现相似水平，而P2X（5）和P2X（6）的水平一致地较低。动脉和静脉之间P2X（4）表达有明显差异。桡动脉和乳内动脉的P2X水平都很低，而隐静脉A的水平高14.6倍（P < 0.0001），接近主要受体亚型的水平。这些数据显示P2X（4）在静脉中的强表达对冠状动脉旁路移植术中使用的血管的选择具有意义，因为P2X（4）参与钙流入内皮细胞，调节血管收缩性，并且在涉及内膜增殖的情况下上调，表明静脉移植物更容易发生动脉粥样硬化。(C)2002爱思唯尔科学爱尔兰有限公司保留所有权利。
Expression levels of the purinergic P2X receptor subunits (P2X(1) to P2X(7)) and P2Y(2) were examined in the endothelial cell layer of internal mammary artery (Ann. Thorac. Surg. 54 (1992) 652), radial artery (Ann. Thorac. Surg. 16 (1973) 111) and saphenous vein (Ann. Thorac. Surg. 20 (1975) 628) samples obtained at surgery for coronary artery bypass grafts using immunohistochemistry and confocal microscopy. Similar levels of P2X(1), P2X(2), P2X(3), P2X(7) and P2Y(2) were found in the endothelial cells in all vessels examined while the levels of P2X(5) and P2X(6) were uniformly lower. A clear difference was measured in P2X(4) expression between arteries and veins. Both radial and internal mammary arteries exhibited very low levels of P2X(4) whereas the level in the saphenous vein A as 14.6 fold higher (P < 0.0001), approaching that of the major receptor subtypes. These data showing strong expression of P2X(4) in veins have implications for the choice of vessels used in coronary artery bypass grafts given that P2X(4) is involved in calcium influx into endothelial cells, modulates blood vessel contractility and is up-regulated in situations involving intima proliferation suggesting vein grafts are more susceptible to developing atherosclerosis. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.