Ex Vivo Uniaxial Tensile Properties of Rat Uterosacral Ligaments
Ex Vivo Uniaxial Tensile Properties of Rat Uterosacral Ligaments
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DOI：
10.1007/s10439-023-03135-y
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发表时间：
2023-01-18
影响因子：
3.8
通讯作者：
De Vita，Raffaella
中科院分区：
文献类型：
--
作者：
Donaldson，Kandace;De Vita，Raffaella
本文介绍了一种新的测试大鼠子宫骶骨韧带（USL）离体拉伸性能的实验方法。仔细解剖USL样本（），以保留其解剖结构附件，并使用定制单轴拉伸试验器械沿其主活载方向（MD）沿着加载。在加载过程中，在MD和垂直方向（PD）的应变图收集使用数字图像相关技术。平均值（± S.E.M.）最大载荷和最大载荷时的位移分别为N和mm。USL被发现是高度异质性的结构，一些标本在MD中经历的菌株低于，而另一些则达到了在中间区域中的菌株。在0.5 kPa应力下，所有试样均达到该值，MD方向的平均应变为，而在5 kPa应力下，21个试样中只有9个达到该值，平均应变增加到。在单轴载荷下，试样在PD中也伸长，其应变比MD中的应变低一个数量级;在0.5 kPa应力下，PD中的平均应变记录为，在5 kPa应力下，PD中的应变为。随着应力的增加，最大主应变的方向几乎保持不变，这表明由于单轴加载，几乎没有发生微观组织重组。本研究通过提供捕获其复杂机械行为的测试方法指南，作为未来研究大鼠模型中USL支持功能的跳板。
This manuscript presents new experimental methods for testing theex vivotensile properties of the uterosacral ligaments (USLs) in rats. The USL specimens () were carefully dissected to preserve their anatomical attachments, and they were loaded along their mainin vivoloading direction (MD) using a custom-built uniaxial tensile testing device. During loading, strain maps in both the MD and the perpendicular direction (PD) were collected using the digital image correlation technique. The mean (± S.E.M.) maximum load and displacement at the maximum load wereN andmm, respectively. The USLs were found to be highly heterogeneous structures, with some specimens experiencing strains in the MD that were lower thanand others reaching strains that were up toin the intermediate region. At 0.5 kPa stress, a value reached by all the specimens, the mean strain in the MD waswhile at 5 kPa stress, a value achieved only by 9 out of the 21 specimens, the mean strain increased to. Under uniaxial loading, the specimens also elongated in the PD, with strains that were one order of magnitude lower than the strains in the MD; at the 0.5 kPa stress, the mean strain in the PD was recorded to beand, at the 5 kPa stress, the strain in the PD was. The directions of maximum principal strains remained almost unchanged with the increase in stress, indicating that little microstructural re-organization occurred due to uniaxial loading. This study serves as a springboard for future investigations on the supportive function of the USLs in the rat model by offering guidelines on testing methods that capture their complex mechanical behavior.