Nucleic acid splicing events occur frequently during macronuclear development in the protozoan Oxytricha nova and involve the elimination of unique DNA.
Nucleic acid splicing events occur frequently during macronuclear development in the protozoan Oxytricha nova and involve the elimination of unique DNA.
复制标题
在原生动物尖毛虫的大核发育过程中，核酸剪接事件频繁发生，并涉及独特 DNA 的消除。
DOI：
10.1101/gad.1.4.323
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发表时间：
1987
影响因子：
10.5
通讯作者：
Klobutcher,LA
中科院分区：
文献类型：
--
作者：
Ribas-Aparicio,RM;Sparkowski,JJ;Proulx,AE;Mitchell,JD;Klobutcher,LA
在其生命周期中，hypotrichous纤毛虫原生动物Oxytricha nova将其染色体微核的拷贝转化为转录活性大核，其仅包含线性的基因大小的DNA分子，平均大小约为2.2个DNA酶对（kbp）。本文研究了两个大核DNA分子的微核前体。每一个被发现包含至少五个在成熟的大核DNA分子中不存在的DNA序列块。这些序列块，称为内部消除序列（IES），必须在发育过程中通过核酸断裂和连接过程去除。迄今为止获得的数据表明，IES是常见的，并表明在大核发育过程中发生了超过60，000个IES去除事件。其他分析表明，IES代表了已知在发育过程中被消除的独特微核DNA序列的一部分。IES序列的比较显示了共同的组织特征和一些有限的主要序列同源性，这表明它们的发育切除模型。
During its life cycle, the hypotrichous ciliated protozoan Oxytricha nova transforms a copy of its chromosomal micronucleus into a transcriptionally active macronucleus which contains exclusively linear, gene-sized DNA molecules with an average size of about 2.2 kilobase pairs (kbp). The micronuclear precursors of two macronuclear DNA molecules have been examined. Each was found to contain at least five blocks of DNA sequences that are absent in the mature macronuclear DNA molecule. These blocks of sequences, referred to as internal eliminated sequences (IESs), must be removed by a nucleic acid breakage and joining process during development. The data obtained to date indicate that IESs are common and suggest that greater than 60,000 IES removal events occur during macronuclear development. Additional analyses indicate that IESs represent a portion of the unique micronuclear DNA sequences known to be eliminated during development. Comparisons of the sequences of IESs revealed common organizational features and some limited primary sequence homologies that suggest models for their developmental excision.