Enhancement of 1,25-dihydroxyvitamin D3- and all-trans retinoic acid-induced differentiation of human leukemia HL-60 cells by Phyllostachys nigra var. henonis
Enhancement of 1,25-dihydroxyvitamin D3- and all-trans retinoic acid-induced differentiation of human leukemia HL-60 cells by Phyllostachys nigra var. henonis
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DOI：
10.1080/08923970701283310
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发表时间：
2007-01-01
影响因子：
3.3
通讯作者：
Yoo, Jin Cheol
中科院分区：
文献类型：
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作者：
Kim, Seung Hyun;Kim, Tae Sung;Yoo, Jin Cheol
人髓性白血病HL-60细胞在分别用1，25-二羟维生素D-3 [1，25-（OH）（2）D-3]或全反式维甲酸（RA）处理时分化为单核细胞或粒细胞系。本研究采用HL-60细胞培养体系，研究竹叶丙酮提取物对HL-60细胞分化的影响。用50-400 μ g/ml竹叶丙酮提取物处理HL-60细胞72小时，MTT和硝基四氮唑蓝还原试验表明，竹叶丙酮提取物抑制HL-60细胞增殖，诱导细胞分化。有趣的是，当竹叶丙酮组分与5 nM 1，25-（OH）（2）D-3或50 nM全反式RA组合时，观察到HL-60细胞分化的协同诱导。流式细胞仪分析表明，1，25-（OH）（2）D-3和竹叶丙酮馏分的组合刺激分化主要为单核细胞，而全反式RA和竹叶丙酮馏分的组合刺激分化主要为粒细胞。这些结果提示竹叶丙酮提取物具有促进白血病细胞分化的作用，提示竹叶在白血病治疗中的可能性。
Human myeloid leukemia HL-60 cells are differentiated into monocytic or granulocytic lineage when treated with 1,25-dihydroxyvitamin D-3 [1,25-(OH)(2)D-3] or all-trans retinoic acid (RA), respectively. In this study, the effect of acetone fraction prepared from bamboo leaf on cell differentiation was investigated in a HL-60 cell culture system. Treatment of HL-60 cells with 50-400 mu g/ml acetone fraction of bamboo leaf for 72 hr inhibited cell proliferation and induced a little increase in cell differentiation, as demonstrated by the MTT and nitroblue tetrazolium reduction assay. Interestingly, synergistic induction of HL-60 cell differentiation was observed when the acetone fraction of bamboo leaf was combined with either 5 nM 1,25-(OH)(2)D-3 or 50 nM all-trans RA. Flow cytometric analysis indicated that combinations of 1,25-(OH)(2)D-3 and the acetone fraction of bamboo leaf stimulated differentiation predominantly to monocytes, whereas combinations of all-trans RA and the acetone fraction of bamboo leaf stimulated differentiation predominantly to granulocytes. These results suggest that the acetone fraction of bamboo leaf enhanced leukemia cell differentiation and suggest a possibility of bamboo in the treatment of leukemia.