Elevated levels of proinflammatory cytokines in the semen of patients with chronic prostatitis chronic pelvic pain syndrome
Elevated levels of proinflammatory cytokines in the semen of patients with chronic prostatitis chronic pelvic pain syndrome
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DOI：
10.1016/s0090-4295(98)00390-2
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发表时间：
1998-11-01
期刊：
影响因子：
2.1
通讯作者：
Hebel, JR
中科院分区：
文献类型：
--
作者：
Alexander, RB;Ponniah, S;Hebel, JR
目标.慢性前列腺炎/慢性骨盆疼痛综合征是一种常见的诊断，但对这种疾病的了解很少。诊断仅基于症状，没有可测量的参数可以帮助确定疾病的存在，其严重程度或其原因。细胞因子是由免疫系统的细胞分泌的可溶性蛋白质，其主要调节炎症和免疫应答。为了提供生殖道炎症的客观测量，我们测量了慢性前列腺炎/慢性盆腔疼痛综合征男性精液中促炎细胞因子、肿瘤坏死因子-α（TNF-α）和白细胞介素-1 β（IL-1 β）的水平，并将其与正常男性的水平进行比较。我们从18名患有慢性前列腺炎/慢性骨盆疼痛综合征的男性和8名正常男性志愿者中获得精液样本。采用双抗体夹心酶联免疫吸附法测定精浆细胞因子水平。前列腺炎患者精浆中IL-1 β和TNF-α的平均水平（平均值± SEM）高于正常男性：TNF-α分别为98 ± 39和17 ± 8; IL-1 β分别为246 ± 63和27 ± 10; P
Objectives. Chronic prostatitis/chronic pelvic pain syndrome is a common diagnosis, but the disease is poorly understood. The diagnosis is based only on symptoms, and no measurable parameter can help in defining the presence of the disease, its severity, or its cause. Cytokines are soluble proteins secreted by cells of the immune system that principally regulate inflammatory and immune responses. To provide an objective measure of inflammation in the genital tract, we measured levels of the proinflammatory cytokines, tumor necrosis factor-alpha (TNF-alpha) and interleukin-l beta (IL-l beta) in the semen of men with chronic prostatitis/chronic pelvic pain syndrome and compared these with levels in normal men.Methods. We obtained semen samples from 18 men with chronic prostatitis/chronic pelvic pain syndrome and from 8 normal male volunteers. Cytokine levels were measured in the seminal plasma by two-antibody enzyme-linked immunosorbent assay.Results. Men with prostatitis had higher mean levels of IL-l beta and TNF-alpha in seminal plasma (mean +/- SEM) than normal men: TNF-alpha 98 +/- 39 versus 17 +/- 8; IL-1 beta 246 +/- 63 versus 27 +/- 10, respectively; P