Characterization of a new member of the TNF family expressed on antigen presenting cells
Characterization of a new member of the TNF family expressed on antigen presenting cells
复制标题
DOI：
10.1515/bc.1999.186
复制
发表时间：
1999-12-01
影响因子：
3.7
通讯作者：
Williams, LT
中科院分区：
文献类型：
--
作者：
Tribouley, C;Wallroth, M;Williams, LT
肿瘤坏死因子家族参与免疫系统的调节，其成员参与了多种生物学事件，如细胞凋亡、细胞增殖、分化和生存。在这里，我们提出了一个新的成员，肿瘤坏死因子超家族成员20(TNFSF20)，我们已经从表达序列标签(EST)数据库中鉴定和鉴定。人类蛋白是一种285个氨基酸的II型跨膜蛋白，与肿瘤坏死因子的胞外结构域有19%的同源性。TNFSF20表达于巨噬细胞-单核细胞系细胞、树突状细胞等抗原提呈细胞表面。经细菌脂多糖(LPS)处理后，TNFSF20在表达细胞表面的表达下调，提示膜结合蛋白被切割，细胞外释放一种可溶性因子。重组TNFSF20的可溶性能诱导静息外周血单核细胞(PBMC)的增殖和活化淋巴细胞的死亡。因此，TNFSF20可能在细胞介导的免疫反应的调节中发挥关键作用。
The TNF family is involved in the regulation of the immune system, and its members have been implicated in a variety of biological events such as apoptosis, cell proliferation, differentiation and survival. Here we present a new member of the TNF family, tumor necrosis factor super family member 20 (TNFSF20) that we have identified from the expressed sequence tag (EST) database and characterized. The human protein is a 285 amino acid long type II transmembrane protein and is 19% homologous to TNF in its extra-cellular domain. TNFSF20 is expressed at the surface of antigen presenting cells such as cells of the macrophage-monocyte lineage and dendritic cells. After treatment with bacterial lipopolysaccharide (LPS), TNFSF20 expression is downregulated at the surface of the expressing cells, suggesting that the membrane-bound protein gets cleaved, and that a soluble factor is released in the extra-cellular compartment. The soluble form of the recombinant TNFSF20 induces proliferation of resting peripheral blood monocytes (PBMC) and cell death of activated lymphocytes. TNFSF20 might therefore play a critical role in the regulation of cell-mediated immune responses.