Assessment of three new parasite lactate dehydrogenase (pan-pLDH) tests for diagnosis of uncomplicated malaria
Assessment of three new parasite lactate dehydrogenase (pan-pLDH) tests for diagnosis of uncomplicated malaria
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DOI：
10.1016/j.trstmh.2007.09.014
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发表时间：
2008-01-01
影响因子：
2.2
通讯作者：
Guthmann, Jean-Paul
中科院分区：
文献类型：
--
作者：
Fogg, Carole;Twesigye, Rogers;Guthmann, Jean-Paul
在乌干达进行了一项研究，以评估三种寄生虫乳酸脱氢酶（pan-pLDH）试验（Vistapan（R）、Carestart（TM）和Parabank）的诊断能力。对疑似疟疾的门诊患者进行了HRP 2试验、Paracheck-Pf（R）和Giemsa染色血片以及pLDH试验。总共招募了460名受试者：248名血膜阳性，212名血膜阴性。恶性疟原虫存在于95%的感染中。两种pLDH检测Carestart（95.6%）和Vistapan（91.9%）的敏感性均高于90%，Parabank（94.3%）和Carestart（91.5%）的特异性均高于90%。低寄生虫血症敏感性降低（X-2趋势，P>= 100/mu l时90%）。所有.测验具有良好的读者间信度（K > 0.95）。诊断后2周pLDH试验仍有4-10%阳性，而HRP 2试验仍有69.7%阳性。所有测试都具有类似的易用性。总之，两种pLDH试验在诊断恶性疟原虫疟疾中表现良好，并且所有pLDH试验在治疗后均比HRP 2更快地变为阴性。因此，在这一领域，青蒿素联合疗法的快速检测选择将是这些新的pLDH检测之一。(c)2007年皇家热带医学和卫生学会。由爱思唯尔有限公司出版。保留所有权利。
A study to assess the diagnostic capabilities of three parasite lactate dehydrogenase (pan-pLDH) tests, Vistapan (R), Carestart (TM) and Parabank', was conducted in Uganda. An HRP2 test, Paracheck-Pf (R), and a Giemsa-stained blood film were performed with the pLDH tests for outpatients with suspected malaria. In total, 460 subjects were recruited: 248 with positive blood films and 212 with negative blood films. Plasmodium falciparum was present in 95% of infections. Sensitivity above 90% was shown by two pLDH tests, Carestart (95.6%) and Vistapan (91.9%), and specificity above 90% by Parabank (94.3%) and Carestart (91.5%). Sensitivity decreased with low parasitaemia (X-2 trend, P90% with parasitaemia >= 100/mu l. All. tests had good inter-reader reliability (K > 0.95). Two weeks after diagnosis, 4-10% of pLDH tests were stilt positive compared with 69.7% of the HRP2 tests. All tests had similar ease of use. In conclusion, two pLDH tests performed well in diagnosing P. falciparum malaria, and all pLDH tests became negative after treatment more quickly than the HRP2. Therefore the rapid test of choice for use with artemisinin-combination therapies in this area would be one of these new pLDH tests. (c) 2007 Royal Society of Tropical Medicine and Hygiene. Published by Elsevier Ltd. All rights reserved.