Characterization of Two Putative Protein Phosphatase Genes and Their Involvement in Phosphorus Efficiency in Phaseolus vulgaris
Characterization of Two Putative Protein Phosphatase Genes and Their Involvement in Phosphorus Efficiency in Phaseolus vulgaris
复制标题
菜豆中两个假定蛋白磷酸酶基因的特征及其与磷效率的关系
DOI：
10.1111/j.1744-7909.2012.01126.x
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发表时间：
2012-06-01
影响因子：
11.4
通讯作者：
Liao, Hong
中科院分区：
文献类型：
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作者：
Liang, Cui-Yue;Chen, Zhi-Jian;Liao, Hong
由蛋白磷酸酶介导的蛋白质去磷酸化在植物响应环境胁迫的信号转导中起着重要作用。在这项研究中，两个假定的蛋白磷酸酶，PvPS 2：1和PvPS 2：2的确定和特点的豆类（菜豆）。通过在洋葱表皮细胞中瞬时表达PvPS 2：GFP，发现这两个PvPS 2成员定位于质膜和细胞核。在两种菜豆基因型G19833（磷高效基因型）和DOR 364（磷低效基因型）中，磷饥饿显著增加了两个PvPS 2基因的转录。然而，G19833表现出更高的PvPS 2：1表达水平比DOR 364在叶片和根在磷饥饿。通过对转基因拟南芥中PvPS 2：1启动子融合β-葡萄糖醛酸酶（GUS）的组织化学分析，进一步证实了PvPS 2：1响应于Pi饥饿的转录增加。对菜豆毛状根和拟南芥中PvPS 2：1过表达株系的分析表明，PvPS 2：1参与了根的生长和磷的积累。此外，在过表达PvPS 2：1的拟南芥品系中，两个磷饥饿响应基因的表达水平上调，AP酶活性增强。综上所述，我们的研究结果强烈表明，PvPS 2：1正调控植物对磷饥饿的反应，并可以进一步作为候选基因，以提高作物磷效率。
Protein dephosphorylation mediated by protein phosphatases plays a major role in signal transduction of plant responses to environmental stresses. In this study, two putative protein phosphatases, PvPS2:1 and PvPS2:2 were identified and characterized in bean (Phaseolus vulgaris). The two PvPS2 members were found to be localized to the plasma membrane and the nucleus by transient expression of PvPS2:GFP in onion epidermal cells. Transcripts of the two PvPS2 genes were significantly increased by phosphate (Pi) starvation in the two bean genotypes, G19833 (a P-efficient genotype) and DOR364 (a P-inefficient genotype). However, G19833 exhibited higher PvPS2:1 expression levels than DOR364 in both leaves and roots during Pi starvation. Increased transcription of PvPS2:1 in response to Pi starvation was further verified through histochemical analysis of PvPS2:1 promoter fusion beta-glucuronidase (GUS) in transgenic Arabidopsis plants. Analysis of PvPS2:1 overexpression lines in bean hairy roots and Arabidopsis showed that PvS2:1 was involved in root growth and P accumulation. Furthermore, expression levels of two Pi starvation responsive genes were upregulated and the APase activities were enhanced in the overexpressing PvPS2:1 Arabidopsis lines. Taken together, our results strongly suggested that PvPS2:1 positively regulated plant responses to Pi starvation, and could be further targeted as a candidate gene to improve crop P efficiency.