Selection of Temperature-sensitive Activating Enzyme Mutants in Escherichia coli
Selection of Temperature-sensitive Activating Enzyme Mutants in Escherichia coli
复制标题
大肠杆菌温度敏感激活酶突变体的筛选
DOI：
10.1128/jb.95.3.991-997.1968
复制
发表时间：
1968
影响因子：
3.2
通讯作者：
D. Anderson
中科院分区：
文献类型：
--
作者：
S. Kaplan;D. Anderson
A method based on temperature-conditional resistance to thymineless death has been designed to facilitate the isolation of amino acid-activating enzyme mutants. This method may be modified to obtain a greater or lesser proportion of activating enzyme mutants. In the latter instance, an increased proportion of temperature-sensitive macromolecule mutants of other types is obtained. Additional uses of this procedure are discussed.
