Biallelic and triallelic approaches of 5-HTTLPR polymorphism are associated with food intake and nutritional status in childhood
Biallelic and triallelic approaches of 5-HTTLPR polymorphism are associated with food intake and nutritional status in childhood
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DOI：
10.1016/j.jnutbio.2017.01.015
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发表时间：
2017-05-01
影响因子：
5.6
通讯作者：
Almeida, Silvana
中科院分区：
文献类型：
--
作者：
Miranda, Raquel C. K.;Genro, Julia P.;Almeida, Silvana
背景：5-i-itt基因启动子区域的插入/缺失(5-Hitler)产生长(L)或短(S)等位基因(双等位基因法)和SNP(Rs25531)(三等位基因法)。目的：采用双等位基因和三等位基因方法研究5-希特勒和rs25531基因多态性与8岁以下儿童膳食摄入量和人体测量学参数的关系。结果：在双等位基因分析中，S/S等位基因携带者在3~4岁和7~8岁的体重指数Z评分和皮皱总和均高于L等位基因携带者。在三等位基因分析中，S/S、LG/S+LG/LG等位基因与1岁儿童较高的能量摄入量和3~4岁的腰围相关。结论：在双等位基因分析中，5-希特勒基因多态性与儿童的摄食量、体重指数Z分和皮褶总数相关，从而加强了5-羟色胺转运体在儿童肥胖中的作用。我们的数据表明，在检测儿童时期食物摄入量和营养状况的相关性方面，双等位基因方法比三等位基因方法更敏感。在生命早期识别易感基因可以为干预生活方式提供基础，以防止儿童成为肥胖的成年人。(C)爱思唯尔公司出版的2017年。
Background: The 5-I-ITT gene contains polymorphisms in its promoter region, the insertion/deletion (5-HITLER) that creates long (L) or short (S) alleles (biallelic approach) and SNP (rs25531) in L allele (triallelic approach).Objectives: The aim of this study is to investigate the association of the 5-HITLER and rs25531 polymorphisms, using bi-and triallelic approach, with dietary intake and anthropometric parameters in children followed until 8 years old.Methods: The sample were 303 children who were recruited at birth and examined at 1, 3 to 4 and 7 to 8 years old. The polymorphisms were analyzed by polymerase-chain-reaction-based methods.Results: In the biallelic approach, children with the S/S genotype presented a higher body mass index Z-score in the three developmental stages and higher sum of skinfolds at 3 to 4 and 7 to 8 years old than carriers of the L allele. In the triallelic approach, S/S, Lg/S plus Lg/Lg genotypes were associated with higher energy intake daily at 1 year old and with waist circumference at 3 to 4 years old.Conclusions: In the biallelic approach, the 5-HITLER polymorphism is associated with food intake, body mass index Z-score and sum of skinfolds in children, reinforcing the role of the serotonin transporter in childhood obesity. Our data indicate that the biallelic approach is more sensible than the triallelic approach for detected associations with food intake and nutritional status in childhood. Identifying susceptibility genes in early life could provide the foundations for interventions in lifestyle to prevent children to become obese adults. (C) 2017 Published by Elsevier Inc.