Dynamics of co-translational protein targeting.
Dynamics of co-translational protein targeting.
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DOI：
10.1016/j.cbpa.2015.09.016
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发表时间：
2015-12
影响因子：
7.8
通讯作者：
Walter P
中科院分区：
文献类型：
--
作者：
Elvekrog MM;Walter P
大多数膜蛋白和分泌蛋白通过信号识别颗粒（SRP）及其受体协同转运到目的膜上的蛋白转运通道。这种共翻译分子机制在所有生命王国中都是保守的，尽管它在组成和功能上有所不同。在这里，我们报告了最近的进展，了解机制的SRP功能，重点是发现有关E。coli SRP在整个靶向过程中的构象动态。这些见解揭示了靶向循环中的一个关键检查点：SRP如何调节积极翻译的核糖体与膜上的易位机制的接合。
Most membrane and secretory proteins are delivered co-translationally to protein translocation channels in their destination membrane by the signal recognition particle (SRP) and its receptor. This co-translational molecular machinery is conserved across all kingdoms of life, though it varies in composition and function. Here we report recent progress towards understanding the mechanism of SRP function, focusing on findings about E. coli SRP’s conformational dynamics throughout the targeting process. These insights shed light on a key checkpoint in the targeting cycle: how SRP regulates engagement of an actively translating ribosome with the translocation machinery at the membrane.